README

## Title of Dataset

Rapid evolution of consumptive and non-consumptive predator effects on prey population densities, bioenergetics and stoichiometry

https://doi.org/10.5061/dryad.qbzkh18r5

## A brief summary of dataset contents, contextualized in experimental procedures and results

A 6-week outdoor mesocosm experiment with Caged-Fish (NCE) and Free-Ranging-Fish (CE and NCE) treatments was conducted to quantify and compare the effects of CE and NCE on population densities, bioenergetic and stoichiometric body contents of Daphnia magna.

The experimental procedures include the outdoor mesocosm culturing period under the three different treatments for 6 weeks, sampling and traits measurements.

The results are: Both Caged-Fish and Free-Ranging-Fish treatments decreased the body size and population densities, especially in Daphnia from the high-fish period. Only the Free-Ranging-Fish treatment affected bioenergetic variables, while both the Caged-Fish and Free-Ranging-Fish treatments shaped body stoichiometry. The effects of CE and NCE were different between both periods indicating their rapid evolution in the natural resurrected population. Both the Caged-Fish and Free-Ranging-Fish treatments changed the clonal frequencies of the experimental Daphnia populations of the pre-fish as well as the high-fish period, indicating that not only CE but also NCE induced clonal sorting, hence rapid evolution during the mesocosm experiment in both periods.

## Description of the data and file structure

This is the raw data of a mesocosm experiment aiming to test the consumptive and non-consumptive effects on two experimental populations of Daphnia magna from two contrasting periods (pre-fish and high-fish) that experienced different fish predation pressure.

This dataset includes different treatments and the raw data of all the measured traits of Daphnia.

There were three treatments: no-fish control, caged-fish treatment and free-ranging fish treatment.

The measured raw data includes information about the size and density, body stoichiometry, energy budget and the clonal composition of these two experimental populations. Each with three replicates.

1) Densities were expressed as number of individuals per liter. The body sizes were measured from the eyesite to the end of the body but except the spine length. Adult body size and average body size were measured on a random subset of 10 adults or of 10 random individuals (including juveniles), respectively, from each sample.

2) body stoichiometry were measured as the the body C%, N% and P%, and their ratios C:N, C:P and N:P.

3) for energy budget, we quantified the available energy reserves (Ea), the energy consumption (Ec) and integrated these to determine the cellular energy allocation (CEA) that was calculated as Ea – Ec.

4) the clonal compositions were for each experimental mesocosm population at the end of the experiment by genotyping 24 randomly chosen individuals from each mesocosm.

## Sharing/Access information

Links to other publicly accessible locations of the data: All data was also deposited in Figshare (DOI: 10.6084/m9.figshare.22324972).

## Code/Software

This dataset has no code.