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Dryad

A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew

Cite this dataset

He, Huagang et al. (2024). A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew [Dataset]. Dryad. https://doi.org/10.5061/dryad.qjq2bvqmz

Abstract

Many disease resistance genes have been introgressed into wheat from its wild relatives. However, reduced recombination within the introgressed segments hinders cloning of the introgressed genes. Here, we report the identification of wheat powdery mildew resistance gene Pm13 originated from from Aegilops longissima using a method combining physical mapping with radiation-induced chromosomal aberrations and transcriptome sequencing analysis of ethyl methanesulfonate (EMS)-induced loss-of-function mutants. Pm13 encodes a kinase fusion protein, designated MLKL-K, with an N-terminal domain of mixed lineage kinase domain-like protein (MLKL_NTD domain) and a C-terminal serine/threonine kinase domain bridged by a brace. The resistance function of Pm13 is validated by transient and stable transgenic complementation assays. Transient over-expression analyses in Nicotiana benthamiana leaves and wheat protoplasts reveal that the fragment Brace-Kinase122-476 of MLKL-K is capable of inducing cell death, which is dependent on a functional kinase domain and the three α-helices in the brace region close to the N-terminus of the kinase domain.

README: Title of Dataset

A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew

Description of the data and file structure

  • The file pm13-wt_assembled_transcriptome_sorted.fasta.gz contains assembled transcriptome of Bgt-infected wt line No.3778 leaves.
  • The file NODE_25350.fa contains sequence of assembled transcript NODE_25350_length_2006_cov_24.294361_g11779_i0 extracted from file pm13-wt_assembled_transcriptome_sorted.fasta.gz. The file Pm13_CDS.fa contains coding sequence of Pm13/MLKL-K.
  • The file Pm13_genomic_sequence.fa contains 11,376 bp genomic sequence of Pm13/MLKL-K isolated from wheat line No.3778.