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Data from: Linking pollen foraging of megachilid bees to their nest bacterial microbiota


Voulgari-Kokota, Anna et al. (2021), Data from: Linking pollen foraging of megachilid bees to their nest bacterial microbiota, Dryad, Dataset,


Solitary bees build their nests by modifying the interior of natural cavities and they provision them with food by importing collected pollen. As a result, the microbiota of the solitary bee nests may be highly dependent on introduced materials. In order to investigate how the collected pollen is associated with the nest microbiota, we used metabarcoding of the ITS2 rDNA and the 16S rDNA to simultaneously characterize the pollen composition and the bacterial communities of 100 solitary bee nest chambers belonging to seven megachilid species. We found a weak correlation between bacterial and pollen alpha-diversity and significant associations between the composition of pollen and that of the nest microbiota, contributing to the understanding of the link between foraging and bacteria acquisition for solitary bees. Since solitary bees cannot establish bacterial transmission routes through eusociality, this link could be essential for obtaining bacterial symbionts for this group of valuable pollinators.

Usage notes

OTU tables and identities of bacteria and pollen

Data tables with taxonomic identities and read abundances in each sample for pollen and larval microbiomes and nest pollens.



Deutsche Forschungsgemeinschaft, Award: KE1743/4-1