Data from: Eocene shark teeth from peninsular Antarctica: Windows to habitat use and paleoceanography
Data files
Aug 05, 2024 version files 210.67 MB
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Larocca_Conte_et._al._2024_-_Shark_FEST_Data_Repositery.zip
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README.md
Sep 16, 2024 version files 210.70 MB
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Larocca_Conte_et._al._2024_-_Shark_FEST_Data_Repositery.zip
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README.md
Abstract
Eocene climate cooling, driven by the falling pCO2 and tectonic changes in the Southern Ocean, impacted marine ecosystems. Sharks in high-latitude oceans, sensitive to these changes, offer insights into both environmental shifts and biological responses, yet few paleoecological studies exist. The Middle-to-Late Eocene units on Seymour Island, Antarctica, provide a rich, diverse fossil record, including sharks. We analyzed the oxygen isotope composition of phosphate from shark tooth bioapatite (δ18Op) and compared our results to co-occurring bivalves and predictions from an isotope-enabled global climate model to investigate habitat use and environmental conditions. Bulk δ18Op values (mean 22.0 ± 1.3‰) show no significant changes through the Eocene. Furthermore, the variation in bulk δ18Op values often exceeds that in simulated seasonal and regional values. Pelagic and benthic sharks exhibit similar δ18Op values across units but are offset relative to bivalve and modeled values. Some taxa suggest movements into warmer or more brackish waters (e.g., Striatolamia, Carcharias) or deeper, colder waters (e.g., Pristiophorus). Taxa like Raja and Squalus display no shift, tracking local conditions in Seymour Island. The lack of difference in δ18Op values between pelagic and benthic sharks in the Late Eocene could suggest a poorly stratified water column, inconsistent with a fully opened Drake Passage. Our findings demonstrate that shark tooth bioapatite tracks the preferred habitat conditions for individual taxa rather than recording environmental conditions where they are found. A lack of secular variation in δ18Op values says more about species ecology than the absence of regional or global environmental changes.
README: Data from: Eocene Shark Teeth from Peninsular Antarctica: Windows to Habitat Use and Paleoceanography.
https://doi.org/10.5061/dryad.qz612jmq2
The repository folder includes scripts and spreadsheets for phosphate oxygen stable isotope (δ18Op) analysis measured from shark tooth biogenic apatite collected from the Eocene deposits of the La Meseta and Submeseta formations (West Antarctica, Seymour Island). It also contains Fourier-Transform Infrared Spectroscopy (FTIR) analysis, a Bayesian model for temperature estimates, and model output extraction scripts from the iCESM simulation for the Early Eocene (Zhu et al., 2020). Scripts and data are stored in specific folders on the type of analysis. All scripts are in R or Python language.
Usage notes
1 "iCESM modeling scripts" directory
The folder includes scripts in Jupiter Notebook format for extracting and plotting iCESM seawater outputs for the Eocene. The folder includes two files: 1) “d18Ow Analysis Script.ipynb” - This is a Python script primarily using the XArray library, to import iCESM output from Zhu et al. (2020), calculating δ18Ow, and reorganizing the output into monthly time intervals along 25 m and 115 m depth slices, while also averaging output down to these depths; 2) “NetCDF Plotting.ipynb” - this is a Python script primarily using the XArray, Matplotlib, and Cartopy libraries. The script writes a single callable function that creates Matplotlib contour plots from iCESM history output. Variables include temperature, salinity, ideal age, oxygen isotopes, and neodymium isotopes, and map projections include Plate Carree, Mollweide, and orthographic (centering on the Drake Passage). Options are built to enable scale normalization or to set maximum and minimum values for data and select colormaps from a predefined selection of Matplotlib’s “Spectral”, “Viridis”, “Coolwarm”, “GNUplot2”, “PiYG”, “RdYlBu”, and “RdYlGn”. For further questions on model output scripts, please email Adam Aleksinski at aaleksin@purdue.edu.
2 "d18O data and maps" directory
The folder includes δ18Op of shark tooth bioapatite and other datasets to interpret shark paleoecology. These datasets include:
· δ18Op of shark tooth bioapatite (“shark FEST d18Op.csv”). Isotope measurements were run at the Stable Isotope Ecosystem Laboratory of (SIELO) University of California, Merced (California, USA).
· Reference silver phosphate material δ18Op for analytical accuracy and precision (“TCEA reference materials.csv"). Isotope measurements were run at the Stable Isotope Ecosystem Laboratory of (SIELO) University of California, Merced (California, USA).
· Bulk and serially sampled δ18Oc data of co-occurring bivalves (Ivany et al., 2008; Judd et al., 2019) (“Ivany et al. 2008_bulk.csv” and “Judd et al., 2019_serial sampling.csv").
· iCESM model temperature and δ18Ow outputs at 3x and 6x pre-industrial CO2 levels for the Early Eocene (Zhu et al., 2020) (“SpinupX3_25m_Mean_Monthly.nc”, “SpinupX6_25m_Mean_Monthly.nc.”, and “CA_x3CO2.csv”). Simulations are integrated from the surface to 25 m.
· δ18O values of invertebrate species published in Longinelli (1965) and Longinelli & Nuti (1973), used to convert bulk δ18Oc (V-SMOW) data of bivalves into δ18Op (V-SMOW) values after δ18Oc (V-PDB) - δ18Oc (V-SMOW) conversion found in Kim et al. (2015) (“d18O carbonate and phosphate references.csv”).
· R script for data analysis ("d18O data and maps.Rmd”). The script provides annotation through libraries, instrumental accuracy and precision tests, tables, statistical analysis, figures, and model output extractions.
. ("TELM_diversity.csv") displays diversity trends of chondrichthyans across TELMs in one of the main figures of the manuscript.
2.1 Dataset description
shark FEST d18Op.csv
· Sample_ID: Identification number of tooth specimens.
· Other_ID: Temporary identification number of tooth specimens.
· Taxon: Species assigned to shark tooth specimens.
· TELM: Stratigraphic units of La Meseta (TELM 2-5; ~45 to ~37 Ma) and Submeseta formations (TELMs 6 and 7; ~37 to ~34 Ma) (Amenábar et al., 2020; Douglas et al., 2014; Montes et al., 2013).
· d18Op: Mean δ18Op values of silver phosphate crystals precipitated from shark tooth bioapatite. Specimens were run in triplicates, corrected, and standardized on the V-SMOW scale.
· sd: Standard deviation of silver phosphate triplicate samples per specimen.
· Protocol: Silver phosphate protocols used to precipitate crystals from shark tooth bioapatite. We adopted the Rapid UC (“UC_Rapid”) and the SPORA (“SPORA”) protocols after Mine et al. and (2017) Larocca Conte et al. (2024) based on the tooth specimen size and sampling strategy. Descriptions of the methods are included in the main manuscript.
· Environment: Inferred shark habitat based on taxonomy classified as benthic or pelagic environment.
· Collection: Institutional abbreviations of museum collections from which shark tooth specimens are housed. NRM-PZ is the abbreviation for the Swedish Natural History Museum (Stockholm, Sweden), PRI is the abbreviation for the Paleontological Research Institute (Ithaca, New York, United States), and UCMP is the University of California Museum of Paleontology (Berkeley, California, United States).
TCEA reference materials.csv
· Identifier_1: unique identifier number per sample.
· sample: reference silver phosphate materials (USGS 80 and USGS 81).
· amount: weight of samples in mg.
· Area 28: peak area of mass 28 (12C16O).
· Area 30: peak area of mass 30 (12C18O).
· d18O_corrected: corrected δ18Op value of reference materials following drift correction, linearity correction, and 2-point calibration to report values on the V-SMOW scale.
Ivany et al. 2008_bulk.csv
· Telm: Stratigraphic units of La Meseta (TELM 2-5; ~45 to ~37 Ma) and Submeseta formations (TELMs 6 and 7; ~37 to ~34 Ma) (Amenábar et al., 2020; Douglas et al., 2014; Montes et al., 2013).
· Locality: Locality code from which bivalves were collected.
· Genus: Genera of bivalves. Specimens are assigned to Cucullaea and Eurhomalea genera.
· Line: Sampling areas of specimens. The sampling strategy is described in Ivany et al. (2008).
· d13C: δ13C values of specimens from sampled lines. Values are reported in the V-PDB scale.
· d18Oc_PDB: δ18Oc values of specimens from sampled lines. Values are reported in the V-PDB scale.
Judd et al., 2019_serial sampling.csv
· Horizon: horizons of the TELM 5 unit (La Meseta Formation) from which bivalves were collected. Horizon 1 is stratigraphically the lowest, while horizon 4 is the highest (Judd et al., 2019).
· ID: Identification number of specimens.
· Latitude: Geographic coordinate where bivalve specimens were collected.
· Longitude: Geographic coordinate where bivalve specimens were collected.
· Surface sampled: Specific sampling area, indicating whether sampling occurred in the interior or exterior portion of shells.
· distance: The distance from the umbo in mm from which sampling occurred along a single shell.
· d18Oc_PDB: δ18Oc values of specimens from sampled areas of shells. Values are reported on the V-PDB scale.
SpinupX3_25m_Mean_Monthly.nc
See section 1 ("iCESM modeling scripts" directory, “d18Ow Analysis Script.ipynb” script) for a full description of the iCESM model output extraction.
SpinupX6_25m_Mean_Monthly.nc
See section 1 ("iCESM modeling scripts" directory, “d18Ow Analysis Script.ipynb” script) for a full description of the iCESM model output extraction.
CA_x3CO2.csv
· lat: Geographic coordinate where temperature and δ18Ow model values are extracted from the iCESM simulation scaled at 3x preindustrial CO2 levels (values averaged within a seawater column depth of 25 m).
· long: Geographic coordinate where temperature and δ18Ow model values are extracted from the iCESM simulation scaled at 3x preindustrial CO2 levels (values averaged within a seawater column depth of 25 m).
· T_mean: Simulated seawater temperature values in °C.
· d18Ow: Simulated seawater δ18Ow values (V-SMOW).
· d18Op: Simulated seawater δ18Op values (V-SMOW). Values were calculated by using seawater temperature and δ18Ow arrays following the paleothermometer equation after Lécuyer et al. (2013).
d18O carbonate and phosphate references.csv
· species: Species of invertebrate taxa.
· type: Specimen type, including barnacles, brachiopods, crabs, and mollusks.
· depth: Depth of seawater column where specimens were collected, reported in meters below sea level when specified.
· d18Op: δ18Op values of invertebrate specimens (V-SMOW).
· d18Oc_PDB: δ18Oc values of invertebrate specimens (V-PDB).
· Reference: Citations from which data were taken to build the dataset (Longinelli, 1965; Longinelli & Nuti, 1973).
TELM diversity.csv
· genus: genera of sharks and rays compiled from literature (Engelbrecht et al., 2016a, 2016b, 2017a, 2017b, 2019; Kriwet, 2005; Kriwet et al., 2016; Long, 1992; Marramá et al., 2018).
· species: species of sharks and rays compiled from literature (Engelbrecht et al., 2016a, 2016b, 2017a, 2017b, 2019; Kriwet, 2005; Kriwet et al., 2016; Long, 1992; Marramá et al., 2018).
· Environment: Inferred shark habitat based on taxonomy classified as benthic or pelagic environment.
· TELM: Stratigraphic units of La Meseta (TELM 1-5; ~44 to ~37 Ma) and Submeseta formations (TELMs 6 and 7; ~37 to ~34 Ma) (Amenábar et al., 2020; Douglas et al., 2014; Montes et al., 2013).
3 “FTIR data” directory
The folder includes FTIR acquisitions and data analysis scripts on reference materials and shark tooth bioapatite for quality checks to test diagenesis effects on δ18Op of sharks. The folder includes:
· The R project file “apatite_ftir.Rproj”. This project file navigates through scripts for raw data processing and data analysis. The background of the raw data was processed following custom R functions from Trayler et al. (2023; https://github.com/robintrayler/collagen_demineralization).
· The “.Rproj.user” folder includes project-specific temporary files (e.g. auto-saved source documents, window-state, etc.) stored by the R project file “apatite_ftir.Rproj”. The folder may be hidden depending on directory view options.
· The “raw data” directory stores spectra acquisitions as .dpt files. Spectra files are stored in the folders “apatite” and “calcite” based on the material type. Spectra were obtained in the 400 – 4000 cm⁻¹ range using a Bruker Vertex 70 Far-Infrared in ATR located at the Nuclear Magnetic Resonance Facility at the University of California Merced (California, USA).
· The “processed” directory includes processed spectra stored as .csv files (“apatite_data.csv” and “calcite_data.csv”) following the background correction (Trayler et al., 2023) and processed infrared data from Larocca Conte et al. (2024) (“Larocca Conte et al._SPORA_apatite_data.csv”) from which the NIST SRM 120c spectrum was filtered. Infrared spectra data in “Larocca Conte et al._SPORA_apatite_data.csv” were obtained and corrected following the same methodologies mentioned above.
· The “R” directory includes R scripts of customized source functions for background correction (Trayler et al., 2023; inspect the "functions" directory and the R script "0_process_data.R") and data analysis (“data_analysis.R”). The scripts provide annotation through libraries and functions used for data processing and analysis.
· Additional datasets. The “data_FTIR_d18O.csv” includes infrared data and δ18Op values of specimens, while the “Grunenwald et al., 2014_CO3.csv” is the dataset after Grunenwald et al. (2014) used to predict carbonate content from the materials featured in this work.
3.1 Dataset description
Spreadsheets included in the “processed” directory
The datasets “apatite_data.csv”, “calcite_data.csv”, and “Larocca Conte et al._SPORA_apatite_data.csv” are structured with the following variables:
· wavenumber: infrared wavenumber in cm-1.
· absorbance: infrared absorbance value.
· file_name: .dpt file name from which infrared wavenumber and absorbance values were obtained following the background correction.
data_FTIR_d18O.csv
· file_name: .dpt file name from which infrared wavenumber and absorbance values were obtained following the background correction.
· v4PO4_565_wavenumber: Wavenumber of maximum infrared absorbance around the first νPO4 band, usually at 565 cm-1.
· v4PO4_565: Peak absorbance value of the first ν4PO4 band (~565 cm-1).
· v4PO4_valley_wavenumber: Wavenumber of valley between ν4PO4 bands.
· v4PO4_valley: Absorbance value of the valley between ν4PO4 bands.
· v4PO4_603_wavenumber: Wavenumber of maximum infrared absorbance around the second ν4PO4 band, usually at 603 cm-1.
· v4PO4_603: Peak absorbance value of the second ν4PO4 band (~603 cm-1).
· CI: Crystallinity index calculated after equation provided in (Shemesh, 1990) as (v4PO4_565 + v4PO4_603 / v4PO4_valley) (i.e., the sum of peak absorbance of νPO4 bands divided by the absorbance value of the valley between peaks).
· material: Material type of samples (i.e., standard material, enameloid, dentin sampled from the crown or root area of shark teeth, and enameloid mixed with dentin).
· AUC_v3PO4: Area under the curve of the ν3PO4 and ν1PO4 bands where maximum absorbance is at ~1025 cm-1 and ~960 cm-1, respectively.
· AUC_v3CO3: Area under the curves of Type-A and Type-B carbonate bands having maximum infrared absorbance at ~1410 (Type-B), ~1456 (Type-B), and ~1545 cm-1 (Type-A).
· v3CO3_v3PO4_ratio: Ratio between area under the curves of carbonate and phosphate bands (i.e., AUC_v3CO3 / AUC_v3PO4).
· CO3_wt: Estimated mean carbonate content following the equation in Grunenwald et al. (2014) (i.e. CO3_wt = 28.4793 (±1.4803) v3CO3_v3PO4_ratio + 0.1808(±0.2710); R2 = 0.985).
· CO3_wt_sd: Standard deviation of estimated carbonate content calculated by propagating the error around coefficients provided in the Grunenwald et al. (2014) equation (see full equation in CO3_wt).
· Taxon: Species assigned to shark tooth specimens.
· TELM: Stratigraphic units of La Meseta (TELM 2-5; ~45 to ~37 Ma) and Submeseta formations (TELMs 6 and 7; ~37 to ~34 Ma) (Amenábar et al., 2020; Douglas et al., 2014; Montes et al., 2013).
· d18Op: Mean δ18Op values of silver phosphate crystals precipitated from shark tooth bioapatite. Specimens were run in triplicates, corrected, and standardized on the V-SMOW scale.
· sd: Standard deviation of silver phosphate triplicate samples per specimen.
· Collection: Institutional abbreviations of museum collections where shark tooth specimens are housed. Infrared spectra were obtained from a selected subset of tooth specimens in the care of the Swedish Natural History Museum (NRM-PZ; Stockholm, Sweden).
Grunenwald et al., 2014_CO3.csv
· sample: Sample code.
· material: Material type of samples (i.e., standard material, bone, and enamel).
· v3CO3: Area under the curves of Type-A and Type-B carbonate bands having maximum infrared absorbance at ~1410 (Type-B), ~1456 (Type-B), and ~1545 cm-1 (Type-A).
· v3PO4: AUC_v3PO4: Area under the curve of the ν3PO4 and ν1PO4 bands where maximum absorbance is at ~1025 cm-1 and ~960 cm-1, respectively.
· v3CO3_v3PO4_ratio: v3CO3_v3PO4_ratio: Ratio between area under the curves of carbonate and phosphate bands (i.e., v3CO3 /v3PO4).
· CO3_wt: Carbonate content measured via CO2 coulometry. Further details about the analytical measurements are found in Grunenwald et al. (2014).
4 “Bayes_FEST_Temperautre Estimates” directory
The folder includes the Bayesian approach used to estimate posterior seawater temperature, δ18Ow values from δ18Op of sharks bioapatite using a Bayesian approach modified after Griffiths et al. (2023). The original scripts used in Griffiths et al. (2023) are reposited here: https://github.com/robintrayler/bayesian_phosphate. The directory includes:
· The R project file “Bayes_FEST.Rproj”. This project file navigates through scripts for raw data analysis.
· The “.Rproj.user” folder includes project-specific temporary files (e.g. auto-saved source documents, window-state, etc.) stored by the R project file “Bayes_FEST.Rproj”. The folder may be hidden depending on directory view options.
· The “data” folder includes the spreadsheets for modeled seawater temperature and δ18Ow values (“CA_x3CO2.csv”) and δ18Op values of shark tooth bioapatite (“shark FEST d18Op.csv”) used as prior information for the Bayesian model. We refer to section 2.1 for the full description of spreadsheets.
· The “R” folder includes customized functions for the Bayesian model stored in the “functions” directory and the script for data analysis (“01_model_sharks.R”). The script includes a comparison of paleothermometer equations after Kolodny et al. (1983), Lécuyer et al. (2013), Longinelli & Nuti (1973), and (Pucéat et al. (2010) using the bulk δ18Op shark tooth bioapatite, simulated seawater temperature and δ18Ow values as prior inputs. While all paleothermometers estimate similar posterior bulk δ18Op close to empirical values, temperature estimates using the Pucéat et al. (2010) method are often the highest, generating estimates ~8°C higher than other equations. We therefore used the Lécuyer et al. (2013) paleothermomether for temperature estimates using δ18Op of shark bioapatite grouped by taxa because it:
1) Provides consistent posterior temperature estimates relative to other equations (Longinelli & Nuti, 1973, Kolodny et al., 1983).
2) provides temperature values from fish tooth specimens consistent with estimates of co-existing bivalves or brachiopod carbonate shells.
The script provides annotation through libraries, statistical analysis, figures, and tables.
4 Software
4.1 R
R and R Studio (R Development Core Team, 2024; RStudio Team, 2024) are required to run scripts included in the "d18O data and maps", “FTIR data”, and “Bayes_FEST_Temperautre Estimates” directories, which were created using versions 4.4.1 and 2024.04.02, respectively. Install the following libraries before running scripts:
“cowplot” (Wilke, 2024), “colorspace” (Zeileis et al., 2020), “DescTools” (Signorell, 2024), “lattice” (Sarkar, 2008), “flextable” (Gohel & Skintzos, 2024), “ggh4x” (van den Brand, 2024), “ggnewscale” (Campitelli, 2024), “ggpubr” (Kassambara, 2023a), “ggspatial” (Dunnington, 2023), “ggstance” (Henry et al., 2024), “ggstar” (Xu, 2022), “greekLetters” (Kévin Allan Sales Rodrigues, 2023), “gridExtra” (Auguie, 2017), “mapdata” (code by Richard A. Becker & version by Ray Brownrigg., 2022); “mapproj” (for R by Ray Brownrigg et al., 2023), “maps” (code by Richard A. Becker et al., 2023), “ncdf4” (Pierce, 2023), “oce” (Kelley & Richards, 2023), “rasterVis” (Oscar Perpiñán & Robert Hijmans, 2023), “RColorBrewer” (Neuwirth, 2022), “rnaturalearth” (Massicotte & South, 2023), “rnaturalearthhires” (South et al., 2024),”rstatix” (Kassambara, 2023b), “scales” (Wickham et al., 2023), “tidyverse” (Wickham et al., 2019), “viridisLite” (Garnier et al., 2023).
4.2 Python
Python scripts, including “d18O Analysis Script.ipynb” and “NetCDF Plotting.ipynb”, utilize the Jupyter Notebook interactive ‘platform and are executed using Python version 3.9.16. Install the following libraries before running scripts:
“xarray” (Hoyer & Joseph, 2017), “matplotlib” (Hunter, 2007), “cartopy” (Met Office, 2015).
5 References
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Methods
See methods in Larocca Conte, G., Aleksinski, A., Liao, A., Kriwet, J., Mörs, T., Trayler, R. B., Ivany, L. C., Huber, M., Kim, S. L. (2024). A proximal perspective to the Eocene Drake Passage: environmental reconstruction and habitat use based on fossil shark teeth from Seymour Island, Antarctica. Paleoceanography and Paleoclimatology (in review).