Transmission electron micrographs of AMPAR containing vesicles

Peters, John, Stanford University, https://orcid.org/0000-0002-3192-7824

Leitz, Jeremy, Stanford University

Oses-Prieto, Juan, University of California, San Francisco

Burlingame, Alma, University of California, San Francisco

Brunger, Axel, Stanford University, https://orcid.org/0000-0001-5121-2036

johnjp@stanford.edu, jleitz@stanford.edu, joses@cgl.ucsf.edu, alb@cgl.ucsf.edu, brunger@stanford.edu

Published Sep 24, 2021 on Dryad. https://doi.org/10.5061/dryad.r2280gbdh

Cite this dataset

Peters, John et al. (2021). Transmission electron micrographs of AMPAR containing vesicles [Dataset]. Dryad. https://doi.org/10.5061/dryad.r2280gbdh

Abstract

Here we present transmission electron micrographs of AMPAR containing vesicles. AMPAR containing vesicles were purified from whole mouse brain via immunoisolation, applied to a copper electron microscopy grid, and stained with uranyl acetate. Vesicles were imaged at a range of magnifications from 4,000 to 15,000. Included in these data are immunoelectron micrographs where we probed for synaptobrevin-2, synaptophysin-1, GluA2, and GluA3.