RNA sequencing for individuals of Daphnia dentifera exposed and unexposed to a fungal pathogen
Data files
Jul 26, 2023 version files 76.08 GB
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Caceres_P1.2016821.tgz
37.11 GB
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Caceres_P2.2016821.tgz
38.97 GB
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README_Daphnia_RNA.txt
3.99 KB
Abstract
This raw sequencing data provided the foundation for the analysis: "Differential gene expression analysis in the aquatic invertebrate, Daphnia dentifera in response to fungal pathogen exposure" by Terrill et al. In this study, authors infected Daphnia dentifera with a commonly occurring fungal pathogen, Metschnikowia bicuspidata, and performed RNA extraction at three different time points (2 hours post-exposure, 12 hours post-exposure, and 24 hours post-exposure). The data presented here include the raw sequencing reads as well as metadata for each sample. After a differential gene expression analysis, the study found that genes related to cuticle development were upregulated in those samples that were exposed to the pathogen when compared to unexposed individuals.
RNA-sequencing data for individuals of Daphnia dentifera, exposed and unexposed to the fungal pathogen Metschnikowia bicuspidata, for differential gene expression analysis. Tissue was extracted using whole-bodies and cDNA libraries were prepared for sequencing using Illumina's TruSeq Stranded mRNA-seq Sample Prep kit and sequenced on a HiSeq 4000 at the University of Illinois at Urbana-Champaign, Illinois: USA. Raw fastq files have been demultiplexed with bcl2fastq.