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RNA sequencing for individuals of Daphnia dentifera exposed and unexposed to a fungal pathogen

Cite this dataset

Terrill, Emily et al. (2023). RNA sequencing for individuals of Daphnia dentifera exposed and unexposed to a fungal pathogen [Dataset]. Dryad. https://doi.org/10.5061/dryad.r7sqv9sfj

Abstract

This raw sequencing data provided the foundation for the analysis: "Differential gene expression analysis in the aquatic invertebrate, Daphnia dentifera in response to fungal pathogen exposure" by Terrill et al. In this study, authors infected Daphnia dentifera with a commonly occurring fungal pathogen, Metschnikowia bicuspidata, and performed RNA extraction at three different time points (2 hours post-exposure, 12 hours post-exposure, and 24 hours post-exposure). The data presented here include the raw sequencing reads as well as metadata for each sample. After a differential gene expression analysis, the study found that genes related to cuticle development were upregulated in those samples that were exposed to the pathogen when compared to unexposed individuals. 

Methods

RNA-sequencing data for individuals of Daphnia dentifera, exposed and unexposed to the fungal pathogen Metschnikowia bicuspidatafor differential gene expression analysis. Tissue was extracted using whole-bodies and cDNA libraries were prepared for sequencing using Illumina's TruSeq Stranded mRNA-seq Sample Prep kit and sequenced on a HiSeq 4000 at the University of Illinois at Urbana-Champaign, Illinois: USA. Raw fastq files have been demultiplexed with bcl2fastq. 

Funding

National Science Foundation