Data from: Phylogenomic insights into the evolution of stinging wasps and the origins of ants and bees
Branstetter, Michael G., University of Utah
Danforth, Bryan N., Utah State University
Pitts, James P., Utah State University
Faircloth, Brant C., Louisiana State University of Alexandria
Ward, Philip S., University of California System
Buffington, Matthew L., Smithsonian Institution
Gates, Michael W., Smithsonian Institution
Kula, Robert R., Smithsonian Institution
Brady, Seán G., Smithsonian Institution
Published Apr 03, 2018 on Dryad.
Cite this dataset
Branstetter, Michael G. et al. (2018). Data from: Phylogenomic insights into the evolution of stinging wasps and the origins of ants and bees [Dataset]. Dryad. https://doi.org/10.5061/dryad.r8d4q
The stinging wasps (Hymenoptera: Aculeata) are an extremely diverse lineage of hymenopteran insects, encompassing over 70,000 described species and a diversity of life history traits, including ectoparasitism, cleptoparasitism, predation, pollen feeding (bees [Anthophila] and Masarinae) and eusociality (social vespid wasps, ants, and some bees) . The most well-studied lineages of Aculeata are the ants, which are ecologically dominant in most terrestrial ecosystems , and the bees, the most important lineage of angiosperm-pollinating insects . Establishing the phylogenetic affinities of ants and bees helps us understand and reconstruct patterns of social evolution as well as fully appreciate the biological implications of the switch from carnivory to pollen feeding (pollenivory). Despite recent advancements in aculeate phylogeny [4–11], considerable uncertainty remains regarding higher level relationships within Aculeata, including the phylogenetic affinities of ants and bees [5–7]. We used ultraconserved element (UCE) phylogenomics [7,12] to resolve relationships among stinging wasp families, gathering sequence data from > 800 UCE loci and 187 samples, including 30 out of 31 aculeate families. We analyzed the 187-taxon data set using multiple analytical approaches, and we evaluated several alternative taxon sets. We also tested alternative hypotheses for the phylogenetic positions of ants and bees. Our results present a highly supported phylogeny of the stinging wasps. Most importantly, we find unequivocal evidence that ants are the sister group to bees+apoid wasps (Apoidea) and that bees are nested within a paraphyletic Crabronidae. We also demonstrate that taxon choice can fundamentally impact tree topology and clade support in phylogenomic inference.
Raw sequence reads.
Raw Illumina reads for all newly sequenced samples from this study.
All alignment supermatrices from this study. In NEXUS format. Includes UCE locus character set information.
BEAST XML data files.
All BEAST XML data files generated for this study.
All phylogenetic trees generated in this study.
All data tables from this study.
Partitioning files for the Hym-187T-F75 alignment supermatrix. Includes bylocus, hcluster, rcluster, and kmeans partitioning schemes. In RAxML format.
Alternative topologies for SH-tests.
All alternative topologies used to test the position of ants and bees. SH-tests were performed using RAxML.
Supplemental figures S1 and S2 combined.
The supplemental figures combined into a single pdf figure.
UCE contigs - aligned, untrimmed, and unfiltered.
Trinity contigs containing UCE loci. The contigs have been extracted from the bulk set of Trinity contigs using Phyluce and aligned, but they have not been trimmed to remove ambiguously aligned sites or filtered to remove loci with missing data.
UCE loci from genome-enabled samples.
UCE loci extracted from available genome-enabled Hymenoptera. Each UCE locus consists of a core region plus 400 bp of flanking DNA on either side. Includes a lastz match-counts database and fasta files for use with Phyluce.
The bulk set of Trinity assemblies for all enriched samples analyzed in this study. Includes assemblies from the previous Faircloth et al. (2015) study. Cleaned reads were assembled using Trinity ver. r2013-02-25.
BaCoCa analysis results. Performed on the Hym-187T-F75 locus set.
National Science Foundation, Award: DEB-1354996, DEB-0814544, DEB-0742998, DEB-1555905