The establishment of neural circuits for female sexual receptivity is essential to reproduction, but the underlying mechanism of associated neurodevelopment is still not fully understood. Here we found that neuropeptide prothoracicotropic hormone (PTTH), hormone of the PG axis, regulates virgin female receptivity through ecdysone in Drosophila melanogaster. PTTH-positive PG neurons are doublesex-expressing neurons, they regulate virgin female receptivity before the metamorphosis during the 3^rd-instar larval stage. Furthermore, the genetic knocking down of ecdysone receptor EcR-A in pC1 neurons regulates the morphological development of pC1 neurons and further the virgin female copulation rate. However, the activity of pC1 neurons did not change, suggesting the changes of synaptic connection between pC1 neurons and other neurons. Analysis of brain transcriptomes when EcR-A was knocked down in pC1 neurons reveals that additional genes are regulated downstream of EcR-A in pC1 neurons. Our work suggests a mechanism underlying how the neurodevelopment of neural circuits regulates virgin female receptivity in adults.