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Data from: Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts

Citation

Raja, Mathan K. et al. (2019), Data from: Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts, Dryad, Dataset, https://doi.org/10.5061/dryad.rn91r08

Abstract

Synaptophysins 1 and 2 and synaptogyrins 1 and 3 constitute a major family of synaptic vesicle membrane proteins. Unlike other widely expressed synaptic vesicle proteins such as vSNAREs and synaptotagmins, the primary function has not been resolved. Here, we report robust elevation in the probability of release of readily releasable vesicles with both high and low release probabilities at a variety of synapse types from knockout mice missing all four family members. Neither the number of readily releasable vesicles, nor the timing of recruitment to the readily releasable pool was affected. The results suggest that family members serve as negative regulators of neurotransmission, acting directly at the level of exocytosis to dampen connection strength selectively when presynaptic action potentials fire at low frequency. The widespread expression suggests that chemical synapses may play a frequency filtering role in biological computation that is more elemental than presently envisioned.

Usage Notes

Keys.tar - This tar archive contains the information needed to make sense of the other archives, which contain the data. Untar and read the Readme.txt file.

Root data directory:

  • Data_replace
  • Figure6C
  • Hippocampus
  • CalyxofHeld
  • Figure6AB_QKO
  • Figure6AB_WT
  • Figure6BD_sygyr3plus
  • Figure6BD_syp1plus
  • Figure6BD_syp1syp2plus
  • Figure6BD_syp2sygr3plus
  • Figure6B_sygr1sygr3plus
  • Figure6B_sygyr1plus
  • Figure6B_syp1sygr1plus
  • Figure6B_syp1sygr3plus
  • Figure6B_syp2plus
  • Figure6B_syp2sygr1plus
  • Keys - contains information in a Readme.txt file that will explain how to access the data.