Data from: One, two or three? Integrative species delimitation of short-range endemic Hemicycla species (Gastropoda: Helicidae) from the Canary Islands based on morphology, barcoding, AFLP and ddRADseq data
Data files
Aug 08, 2024 version files 38.99 MB
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Data_set_01_Hemicycla_COI1_16S.nexus
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Data_set_02_AFLP.nex
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Data_set_03_AFLPred.nex
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Data_set_4_RADseq_SNP_all_polymorphic_positions.nex
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Data_set_5_RADseq_SNPs_one_random_bialleic_SNP_per_locus.nex
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Data_set_6_RADseq_SNPS_one_randam_biallelic_per_locus_present_in_all_pops_mascaensis_snapp.nexus
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Data_set_7_all_loci_alleles.fas
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Data_set_8_BPP.txt
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README.md
Abstract
Hemicycla mascaensis and H. diegoi are short-range endemics that occur allopatrically in small areas in the Teno Mountains in the western part of Tenerife (Canary Islands). Both taxa have been recognised as distinct species based on differences in shell morphology and genital anatomy. Preliminary molecular analyses using mitochondrial markers suggested a potential paraphyly of H. diegoi with regard to H. mascaensis. We here use multilocus AFLP data and ddRADseq data as well as distribution data, data on shell morphology and genital anatomy to assess the status of these taxa using phylogenetic analyses, species tree reconstruction, and molecular species delimitation based on the multispecies coalescent as implemented in BFD* and BPP in an integrative approach. Our analyses show that, based on the analysis of multilocus data, the two taxa are reciprocally monophyletic. Species delimitation methods, however, tend to recognise all investigated populations as distinct species, albeit neither lending unambiguous support to any of the species hypothesis. The comparison of the anatomy of distal genital organs further suggests differentiation within H. mascaensis. This highlights the need for a balanced weighting of arguments from different lines of evidence to determine species status and calls for cautious interpretations of the results of molecular species delimitation analyses, especially in organisms with low active dispersal capacities and expected distinct population structuring such as land snails. Taking all available evidence into account, we favour to recognise H. mascaensis and H. diegoi as distinct species, acknowledging, though, that the recognition of both taxa as subspecies (with possibly a third yet undescribed) would also be an option as morphological differentiation is within the limits of other land snail species that are traditionally subdivided into subspecies.
README: Data from: One, two or three? Integrative species delimitation of short-range endemic Hemicycla species (Gastropoda: Helicidae) from the Canary Islands based on morphology, barcoding, AFLP and ddRADseq data
https://doi.org/10.5061/dryad.rr4xgxd7k
Description of the data and file structure
Files and variables
File: Data_set_01_Hemicycla_COI1_16S.nexus
Description: Aligned partial sequences of the mitochondrial cytochrome c oxidase subunit 1 (cox1) (alignment positions 1-655) and 16S rRNA (alignment positions 656-1,488) genes in nexus format.
File: Data_set_02_AFLP.nex
Description: AFLP loci obtained from 15 specimens belonging to the genus Hemicycla in nexus format.
File: Data_set_03_AFLPred.nex
Description: AFLP loci obtained from 9 specimens belonging to the Hemicycla mascaensis complex in nexus format.
File: Data_set_4_RADseq_SNP_all_polymorphic_positions.nex
Description: Concatenated sequences of 5,454 ddRAD loci with a total of 61,809 SNPs (IUPAC-coded) obtained from 11 specimens belonging to the genus Hemicycla in nexus format.
File: Data_set_5_RADseq_SNPs_one_random_bialleic_SNP_per_locus.nex
Description: Data set in nexus format including one bialleci SNP per locus selected randomly from the 5,454 loci in Data Set 4.
File: Data_set_6_RADseq_SNPS_one_randam_biallelic_per_locus_present_in_all_pops_mascaensis_snapp.nexus
Description: Data set in nexus format including one bialleci SNP per locus selected randomly from 15,297 ddRAD loci obtained from 7 specimens belonging to the Hemicycla mascaensis complex.
File: Data_set_7_all_loci_alleles.fas
Description: Sequence data for 5,482 ddRAD loci obtained from 11 specimens belonging to the genus Hemicycla in fasta format.
File: Data_set_8_BPP.txt
Description: Data set including 300 randomly selected, polymorphic loci from Data set 7 obtained for specimens belonging to the H. mascaensis complex in BPP input format.