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Dryad

Comparative RepeatExplorer analysis of Sorghum purpureosericeum B chromosome positive/negative plant

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Jun 07, 2021 version files 477.84 MB

Abstract

B chromosomes are supernumerary, dispensable chromosomes, the presence of which has been detected in all major groups of living organisms - animals, plants and fungi. They do not pair with A chromosomes during meiosis and have irregular modes of inheritance. Here, we isolated and sequenced DNA from B positive and B negative nuclei of Sorghum purpureosericeum and performed repeat analysis on Illumina raw sequence data. The sequence reads were analysed using RepeatExplorer pipeline in a comparative fashion. The analysis revealed nine putative B specific clusters, which were further characterized and used in order to develop B chromosome specific markers. This dataset provides access to reads used in the analysis; assembled repetitive clusters; and result of comparative analysis between B positive and B negative samples.