https://doi.org/10.5061/dryad.rxwdbrvfh

1. Analysis of gut microbiota by 16S rRNA gene sequencing following with nicotine administration by intraperitoneal injection. At 7 weeks of age, the male mice with comparable average body weight were divided into two groups: the normal diet (ND) group and the high-fat diet (HFD) group (D12492, 60% kcal fat; Research Diets, Inc., NJ, USA) and received twice daily injections of nicotine (0.75 mg/kg body weight, intraperitoneal administration) or saline for 4 weeks.
2. Analysis of gut microbiota by 16S rRNA gene sequencing following with nicotine administration by drinking water. At 7 weeks of age, the male mice received a nicotine solution (200 mg/mL in 2% saccharin vehicle) in drinking water during the 4-week period of HFD exposure.

The V4 region of the 16S rRNA gene was amplified using dual-indexed primers. Sequencing of the amplicons was carried out on an Illumina MiSeq instrument with a MiSeq Reagent Kit V3 (Illumina, San Diego, CA, USA).

Description of the data and file structure

1) Analysis of gut microbiota by 16S rRNA gene sequencing followed with nicotine administration by intraperitoneal injection

1) Fecal DNA from mice fed normal diet (ND)

Vehicle control group (n = 8)

DRR493554

• DRR493554_1.fastq.bz2
• DRR493554_2.fastq.bz2

DRR493555

• DRR493555_1.fastq.bz2
• DRR493555_2.fastq.bz2

DRR493556

• DRR493556_1.fastq.bz2
• DRR493556_2.fastq.bz2

DRR493557

• DRR493557_1.fastq.bz2
• DRR493557_2.fastq.bz2

DRR493558

• DRR493558_1.fastq.bz2
• DRR493558_2.fastq.bz2

DRR493559

• DRR493559_1.fastq.bz2
• DRR493559_2.fastq.bz2

DRR493560

• DRR493560_1.fastq.bz2
• DRR493560_2.fastq.bz2

DRR493561

• DRR493561_1.fastq.bz2
• DRR493561_2.fastq.bz2

Nicotine administered group (n = 8)

DRR493562

• DRR493562_1.fastq.bz2
• DRR493562_2.fastq.bz2

DRR493563

• DRR493563_1.fastq.bz2
• DRR493563_2.fastq.bz2

DRR493564

• DRR493564_1.fastq.bz2
• DRR493564_2.fastq.bz2

DRR493565

• DRR493565_1.fastq.bz2
• DRR493565_2.fastq.bz2

DRR493566

• DRR493566_1.fastq.bz2
• DRR493566_2.fastq.bz2

DRR493567

• DRR493567_1.fastq.bz2
• DRR493567_2.fastq.bz2

DRR493568

• DRR493568_1.fastq.bz2
• DRR493568_2.fastq.bz2

DRR493569

• DRR493569_1.fastq.bz2
• DRR493569_2.fastq.bz2

2)  Fecal DNA from mice fed high-fat diet (HFD)

Vehicle control group (n = 8)

DRR493570

• DRR493570_1.fastq.bz2
• DRR493570_2.fastq.bz2

DRR493571

• DRR493571_1.fastq.bz2
• DRR493571_2.fastq.bz2

DRR493572

• DRR493572_1.fastq.bz2
• DRR493572_2.fastq.bz2

DRR493573

• DRR493573_1.fastq.bz2
• DRR493573_2.fastq.bz2

DRR493574

• DRR493574_1.fastq.bz2
• DRR493574_2.fastq.bz2

DRR493575

• DRR493575_1.fastq.bz2
• DRR493575_2.fastq.bz2

DRR493576

• DRR493576_1.fastq.bz2
• DRR493576_2.fastq.bz2

DRR493577

• DRR493577_1.fastq.bz2
• DRR493577_2.fastq.bz2

Nicotine administered group (n = 8)

DRR493578

• DRR493578_1.fastq.bz2
• DRR493578_2.fastq.bz2

DRR493579

• DRR493579_1.fastq.bz2
• DRR493579_2.fastq.bz2

DRR493580

• DRR493580_1.fastq.bz2
• DRR493580_2.fastq.bz2

DRR493581

• DRR493581_1.fastq.bz2
• DRR493581_2.fastq.bz2

DRR493582

• DRR493582_1.fastq.bz2
• DRR493582_2.fastq.bz2

DRR493583

• DRR493583_1.fastq.bz2
• DRR493583_2.fastq.bz2

DRR493584

• DRR493584_1.fastq.bz2
• DRR493584_2.fastq.bz2

DRR493585

• DRR493585_1.fastq.bz2
• DRR493585_2.fastq.bz2

2) Analysis of gut microbiota by 16S rRNA gene sequencing following with nicotine administration by drinking water

1) Vehicle control group (n = 8)

DRR493586

• DRR493586_1.fastq.bz2
• DRR493586_2.fastq.bz2

DRR493587

• DRR493587_1.fastq.bz2
• DRR493587_2.fastq.bz2

DRR493588

• DRR493588_1.fastq.bz2
• DRR493588_2.fastq.bz2

DRR493589

• DRR493589_1.fastq.bz2
• DRR493589_2.fastq.bz2

DRR493590

• DRR493590_1.fastq.bz2
• DRR493590_2.fastq.bz2

DRR493591

• DRR493591_1.fastq.bz2
• DRR493591_2.fastq.bz2

DRR493592

• DRR493592_1.fastq.bz2
• DRR493592_2.fastq.bz2

DRR493593

• DRR493593_1.fastq.bz2
• DRR493593_2.fastq.bz2

2) 2% Saccharin group (n = 8)

DRR493594

• DRR493594_1.fastq.bz2
• DRR493594_2.fastq.bz2

DRR493595

• DRR493595_1.fastq.bz2
• DRR493595_2.fastq.bz2

DRR493596

• DRR493596_1.fastq.bz2
• DRR493596_2.fastq.bz2

DRR493597

• DRR493597_1.fastq.bz2
• DRR493597_2.fastq.bz2

DRR493598

• DRR493598_1.fastq.bz2
• DRR493598_2.fastq.bz2

DRR493599

• DRR493599_1.fastq.bz2
• DRR493599_2.fastq.bz2

DRR493600

• DRR493600_1.fastq.bz2
• DRR493600_2.fastq.bz2

DRR493601

• DRR493601_1.fastq.bz2
• DRR493601_2.fastq.bz2

3) 2% Saccharin with 200 μg/mL nicotine group (n = 8)

DRR493602

• DRR493602_1.fastq.bz2
• DRR493602_2.fastq.bz2

DRR493603

• DRR493603_1.fastq.bz2
• DRR493603_2.fastq.bz2

DRR493604

• DRR493604_1.fastq.bz2
• DRR493604_2.fastq.bz2

DRR493605

• DRR493605_1.fastq.bz2
• DRR493605_2.fastq.bz2

DRR493606

• DRR493606_1.fastq.bz2
• DRR493606_2.fastq.bz2

DRR493607

• DRR493607_1.fastq.bz2
• DRR493607_2.fastq.bz2

DRR493608

• DRR493608_1.fastq.bz2
• DRR493608_2.fastq.bz2

DRR493609

• DRR493609_1.fastq.bz2
• DRR493609_2.fastq.bz2