Published Nov 25, 2019 on Dryad.
Cite this dataset
Herrera, Carlos M.; Medrano, Mónica; Bazaga, Pilar (2019). Data from: Variation in DNA methylation transmissibility, genetic heterogeneity and fecundity-related traits in natural populations of the perennial herb Helleborus foetidus [Dataset]. Dryad. https://doi.org/10.5061/dryad.s04v1
Inferences about the role of epigenetics in plant ecology and evolution are mostly based on studies of cultivated or model plants conducted in artificial environments. Insights from natural populations, however, are essential to evaluate the possible consequences of epigenetic processes in biologically realistic scenarios with genetically and phenotypically heterogeneous populations. Here we explore associations across individuals between DNA methylation transmissibility (proportion of methylation-sensitive loci whose methylation status persists unchanged after male gametogenesis), genetic characteristics (assessed with AFLP markers), seed size variability (within-plant seed mass variance), and realized maternal fecundity (number of recently recruited seedlings), in three populations of the perennial herb Helleborus foetidus along a natural ecological gradient in southeastern Spain. Plants (sporophytes) differed in the fidelity with which DNA methylation was transmitted to descendant pollen (gametophytes). This variation in methylation transmissibility was associated with genetic differences. Four AFLP loci were significantly associated with transmissibility and accounted collectively for about 40% of its sample-wide variance. Within-plant variance in seed mass was inversely related to individual transmissibility. Number of seedlings recruited by individual plants was significantly associated with transmissibility. The sign of the relationship varied between populations, which points to environment-specific, divergent phenotypic selection on epigenetic transmissibility. Results support the view that epigenetic transmissibility is itself a phenotypic trait whose evolution may be driven by natural selection, and suggest that in natural populations epigenetic and genetic variation are two intertwined, rather than independent, evolutionary factors.
AFLP profiles for 60 plants of Helleborus foetidus from three locations in the Sierra de Cazorla, southeastern Spain, used in the study.
MSAP profiles for paired leaf and pollen samples from 60 plants of Helleborus foetidus from three locations in the Sierra de Cazorla, southeastern Spain, sampled for the study
Seed and seedling data
Mean seed mass, seed mass variance, and number of seedlings occurring within a 0.5 m radius, for the 60 individual plants of Helleborus foetidus from three locations in Sierra de Cazorla, southeastern Spain, sampled for the study.
Microsatellite profiles for putative maternal parents and associated seedlings in the sample of Helleborus foetidus plants studied