Skip to main content
Dryad logo

Spatial structure affects phage efficacy in infecting dual-strain biofilms of Pseudomonas aeruginosa

Citation

Testa, Samuele et al. (2019), Spatial structure affects phage efficacy in infecting dual-strain biofilms of Pseudomonas aeruginosa, Dryad, Dataset, https://doi.org/10.5061/dryad.sj300pb

Abstract

Bacterial viruses, or phage, are key members of natural microbial communities. Yet much research on bacterial-phage interactions has been conducted in liquid cultures involving single bacterial strains. Here we explored how bacterial diversity affects the success of lytic phage in structured communities. We infected a susceptible Pseudomonas aeruginosa strain PAO1 with a lytic phage Pseudomonas 352 in the presence versus absence of an insensitive P. aeruginosa strain PA14, in liquid culture versus colonies on agar. We found that both in liquid and in colonies, inter-strain competition reduced resistance evolution in the susceptible strain and decreased phage population size. However, while all sensitive bacteria died in liquid, bacteria in colonies could remain sensitive yet escape phage infection, due mainly to reduced growth in colony centers. In sum, spatial structure can protect bacteria against phage infection, while the presence of competing strains reduces the evolution of resistance to phage.

Usage Notes