Aquatain Mosquito Formulation (AMF) is a surfactant that spreads across the surface of water bodies to produce a monomolecular film. This study experimentally evaluates the temporal efficacy of AMF against aquatic stages of Anopheles arabiensis and Ochlerotatus caspius under laboratory conditions. Using the recommended application does of 1ml/m², a large species specific difference in the median lethal time for L3-L4 larvae was observed. The median lethal time to 50% mortality (LT50) and 90% mortality (LT90) was 1.3 hours, 95% CI [1.2, 1.4] and 3.8 hours, 95% CI [3.6, 4.0] respectively for Oc. caspius. The corresponding values for An. arabiensis was 8.1 hours, 95% CI [7.3, 9.0] and 59.6 hours, 95% CI [48.5, 76.2]. Based on data from published laboratory studies for a total of seven mosquito species drawn from four genera results in the following three groups, [LT50=1-2 hours, Culex quinquefasciatus, Ochlerotatus caspius] [LT50=8-24, hours, Anopheles minimus, Anopheles arabiensis, Anopheles gambiae s.s] and [LT50 =72-143 hours, Anopheles stephensi, Aedes aegypti,]. In all experiments 100% mortality was achieved given sufficient time. The potential relevance of mortality rate estimates in the context of other studies on the use of monomolecular films for the control of malaria and arbovirus diseases is discussed.

Raw data from experiments and data scraped from published graphs.

Excel file with all the data for the paper.