Data from: Tissue-specific O-GlcNAcylation profiling identifies substrates in translational machinery in the Drosophila mushroom body contributing to olfactory learning
Data files
Apr 12, 2024 version files 102.19 MB
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DisciplineSpecificMetadata.json
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Figure_source_data.zip
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README.md
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Supplementary_file.zip
Abstract
O-GlcNAcylation is a dynamic post-translational modification that diversifies the proteome. Its dysregulation is associated with neurological disorders that impair cognitive function, and yet identification of phenotype-relevant candidate substrates in a brain-region-specific manner remains unfeasible. By combining an O-GlcNAc binding activity derived from Clostridium perfringens OGA (CpOGA) with TurboID proximity labeling in Drosophila, we developed an O-GlcNAcylation profiling tool that translates O-GlcNAc modification into biotin conjugation for tissue-specific candidate substrates enrichment. We mapped the O-GlcNAc interactome in major brain regions of Drosophila and found that components of the translational machinery, particularly ribosomal subunits, were abundantly O-GlcNAcylated in the mushroom body of Drosophila brain. Hypo-O-GlcNAcylation induced by ectopic expression of active CpOGA in the mushroom body decreased local translational activity, leading to olfactory learning deficits that could be rescued by dMyc overexpression-induced increase of protein synthesis. Our study provides a useful tool for future dissection of tissue-specific functions of O-GlcNAcylation in Drosophila and suggests a possibility that O-GlcNAcylation impacts cognitive function via regulating regional translational activity in the brain.
README: Data from: Tissue-specific O-GlcNAcylation profiling identifies substrates in translational machinery in the Drosophila mushroom body contributing to olfactory learning
This dataset includes the data in the above publication. In this publication, we developed an O-GlcNAcylation profiling tool that translates O-GlcNAc modification into biotin conjugation for tissue-specific candidate substrate enrichment. We mapped the O-GlcNAc interactome in major brain regions of Drosophila and found that components of the translational machinery, particularly ribosomal subunits, were abundantly O-GlcNAcylated in the mushroom body of Drosophila brain. Hypo-O-GlcNAcylation induced by ectopic expression of active *Cp*OGA in the mushroom body decreased local translational activity, leading to olfactory learning deficits that could be rescued by dMyc overexpression-induced increase of protein synthesis.
Description of the data and file structure
Files:
1. Figure source data: This file contains the original files and data for all the figures in the article (This includes raw data from Western blot, immunofluorescence, QPCR, statistical graphs, behavioral experiments, GO analysis, and more).
2. Supplementary file: This file contains 11 supplementary files in the article.
Sharing/Access information:
1. The accession numbers for the mass spectrometry data were PXD040547 and PXD040412 on the ProteomeXchange Consortium PRIDE partner repository.
2. We created an online database for tissue-specific O-GlcNAcylation Atlas of Drosophila Brain (tsOGA, link in Related Works) to host these datasets.