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Disentangling the effects of methanogen community and environment on peatland greenhouse gas production by a reciprocal transplant experiment

Citation

Juottonen, Heli (2020), Disentangling the effects of methanogen community and environment on peatland greenhouse gas production by a reciprocal transplant experiment, Dryad, Dataset, https://doi.org/10.5061/dryad.sn02v6x16

Abstract

1. Northern peatlands consist of a mosaic of peatland types that vary spatially and temporally and differ in their methane (CH4) production. Microbial community composition and environment both potentially control the processes that release carbon from anoxic peat either as CH4 or carbon dioxide (CO2), a less potent greenhouse gas than CH4. However, the respective roles of these controls remain unclear, which prevents incorporating microbes in the predictions of peatland CH4 emissions.

2. Here, a reciprocal transplant experiment was carried out to separate the influences of microbial community and environment in CH4 and anaerobic CO2 production. Peat from an acidic Sphagnum bog and a sedge fen with higher pH was enclosed in membrane bags with a pore size of 0.2 µm, preventing microbial colonization from the outside, and transplanted in the field for two months.

3. Potential CH4 production was primarily controlled by the environment. The conditions in the bog suppressed the initially higher activity of fen methanogens and reduced CH4 production by 79%. Against expectations, the inhibition was not specific to acetate-using Methanotrichaceae. Reciprocal transplantation favoured Methanosarcinaceae and potentially methylotrophic methanogenesis in general. Bog methanogens, mostly hydrogenotrophic Methanoregulaceae, retained their community structure and activity in the fen with a slight increase (+37%) in CH4 production.

4. Anaerobic CO2 production was controlled by both the microbial community and the environment. Transplantation led to increased CO2 production in both bog (+50%) and fen peat (+57%) with distinct bacterial community, showing that the new environment directed more carbon to other anaerobic processes than methanogenesis. 5. Taken together, these results relate differences in CH4 production of bogs and fens to ecophysiology of specific methanogen groups. The sensitiveness of fen methanogens to the acidic conditions in Sphagnum bogs can help explain the decrease of CH4 emission in the typical boreal peatland succession from young fens to older bogs. Increase in anaerobic CO2 vs. CH4 production with transplantation shows that disturbances of boreal peatlands can activate poorly defined pathways of anaerobic decomposition.

Methods

This dataset includes data from a reciprocal transplant experiment in the field between a bog and a fen in the boreal Lakkasuo peatland complex in Finland. Peat was transplanted from the bog to the fen and from the fen to the bog for 57 days. At the end of the experiment, the transplanted peat samples and control transplants within the bog and within the fen were analyzed in the laboratory for methane production potential, anaerobic carbon dioxide production potential, pH, number of mcrA transcripts by quantitative PCR (file data1), and methanogen communities by RNA-based mcrA T-RFLP (file data2). In addition, untransplanted native peat at the beginning and the end of the experiment was analyzed. For details of the methods, please see Juottonen (2020).

Usage Notes

Variables in the dataset are explained in the readme file.

Funding

Academy of Finland, Award: 133743

Emil Aaltosen Säätiö