Data from: Reproductive workers show queen-like gene expression in an intermediately eusocial insect, the buff-tailed bumble bee Bombus terrestris
Harrison, Mark C., University of Leicester
Hammond, Rob L., University of Leicester
Mallon, Eamonn B., University of Leicester
Hammond, Robert L., University of Leicester
Published May 07, 2015 on Dryad.
Cite this dataset
Harrison, Mark C.; Hammond, Rob L.; Mallon, Eamonn B.; Hammond, Robert L. (2015). Data from: Reproductive workers show queen-like gene expression in an intermediately eusocial insect, the buff-tailed bumble bee Bombus terrestris [Dataset]. Dryad. https://doi.org/10.5061/dryad.sp048
Bumble bees represent a taxon with an intermediate level of eusociality within Hymenoptera. The clear division of reproduction between a single founding queen and the largely sterile workers is characteristic for highly eusocial species, whereas the morphological similarity between the bumble bee queen and the workers is typical for more primitively eusocial hymenopterans. Also, unlike other highly eusocial hymenopterans, division of labour among worker subcastes is plastic and not predetermined by morphology or age. We conducted a differential expression analysis based on RNA-seq data from 11 combinations of developmental stage and caste to investigate how a single genome can produce the distinct castes of queens, workers and males in the buff-tailed bumble bee Bombus terrestris. Based on expression patterns, we found males to be the most distinct of all adult castes (2411 transcripts differentially expressed compared to nonreproductive workers). However, only relatively few transcripts were differentially expressed between males and workers during development (larvae: 71 and pupae: 162). This indicates the need for more distinct expression patterns to control behaviour and physiology in adults compared to those required to create different morphologies. Among female castes, reproductive workers and their nonreproductive sisters displayed differential expression in over ten times more transcripts compared to the differential expression found between reproductive workers and their mother queen. This suggests a strong shift towards a more queenlike behaviour and physiology when a worker becomes fertile. This contrasts with eusocial species where reproductive workers are more similar to nonreproductive workers than the queen.
Phylip input file for neighbour joining tree
Phylip tree file
Phylip output file for neighbour joining tree
Alignment file for sample MA7: male adults, colony 7
Alignment file for sample MA9: male adults, colony 9
Alignment file for sample MA11: male adults, colony 11
Alignment file for sample ML7: male larvae, colony 7
Alignment file for sample ML9: male larvae, colony 9
Alignment file for sample ML11: male larvae, colony 11
Alignment file for sample MP7: male pupae, colony 7
Alignment file for sample MP9: male pupae, colony 9
Alignment file for sample MP11: male pupae, colony 11
Alignment file for sample MQ7: adult mother queen, colony 7
Alignment file for sample MQ8: adult mother queen, colony 8
Alignment file for sample MQ9: adult mother queen, colony 9
Alignment file for sample QL7: queen larvae, colony 7
Alignment file for sample QP7: queen pupae, colony 7
Alignment file for sample VQ7: adult virgin queen, colony 7
Alignment file for sample WAr7: adult reproductive worker, colony 7
Alignment file for sample WAr8: adult reproductive worker, colony 8
Alignment file for sample WAr9: adult reproductive worker, colony 9
Alignment file for sample WAu8: adult worker with unknown reproductive status, colony 8. This was sample was later categorised as non-reproductive.
Alignment file for sample WAu9: adult worker with unknown reproductive status, colony 9. This was sample was later categorised as reproductive.
Alignment file for sample WAu11: adult worker with unknown reproductive status, colony 11. This was sample was later categorised as non-reproductive.
Alignment file for sample WL7: worker larvae, colony 7
Alignment file for sample WL8: worker larvae, colony 8
Alignment file for sample WL9: worker larvae, colony 9
Alignment file for sample WP7: worker pupae, colony 7
Alignment file for sample WP8: worker pupae, colony 8
Alignment file for sample WP9: worker pupae, colony 9
Contains raw read counts with transcript names as row headers and sample names as column headers
Reduced read count list
All transcripts with 50 or less mapped reads have been removed. This file was used for all differential expression analyses. See DESeq script
This is a standard script taken from the DESeq manual and was used for all differential expression analyses. It should be run within R and requires 'Design' and 'Counts' files.
This file is required for DESeq script and describes sampling design.