Skip to main content
Dryad logo

Factorial design assisted RP-HPLC method for simultaneous determination of fluconazole, itraconazole, and terbinafine

Citation

Roshdy, Aya (2021), Factorial design assisted RP-HPLC method for simultaneous determination of fluconazole, itraconazole, and terbinafine, Dryad, Dataset, https://doi.org/10.5061/dryad.sqv9s4n2t

Abstract

A 23 full factorial design model was utilized for development of a new HPLC method with UV detection to estimate three antifungal drugs simultaneously. Fluconazole (FLU), itraconazole (ITR) and terbinafine (TRH), which are co-administered for severe fungal infections have been determined using MOS-1 Hypersil C18 column and an isocratic eluent; methanol 95% and phosphate buffer 5% with 0.001% triethylamine. The pH adjusted 7, and flow rate of  0.7 mL/min. The three drugs were separated within less than 7 minutes at 210 nm. The developed method gave linear response over 5-80, 5-50 and 1-50 μg/mL for FLU, ITR and TRH, respectively. It showed detection limits of 0.8761, 0.293 and 0.1965 μg/mL and quantification limits of 2.655, 0.8788 and 0.5956 μg/mL for the three drugs, respectively. The design of experiment facilitated the optimization of different variables affecting the separation of the three drugs. The designed method sensitivity permitted the simultaneous estimation of ITR and TRH in spiked human plasma successfully.

Methods

Laboratory work was carried out by A.R. in addition to data analysis and drafting the manuscript.

H.E. and S.S. contributed to the study design and the statistics.

A.E.-B. organized the overall study.

All authors permitted the publication of the manuscript.