This dataset is a "part I" extension of the "Engineered cardiac microbundle time-lapse microscopy image dataset" and contains 732 experimental time-lapse image sequences of beating hiPSC-based cardiac microbundles using microbundle strain gauge platforms [1] ("Type1"). In the "part II" extension, we included 808 experimental time-lapse image sequences of beating hiPSC-based cardiac microbundles using FibroTUG platforms [2] ("Type2"). 

References:

[1] Zhang, K., Cloonan, P. E., Sundaram, S., Liu, F., Das, S. L., Ewoldt, J. K., ... & Chen, C. S. (2021). Plakophilin-2 truncating variants impair cardiac contractility by disrupting sarcomere stability and organization. Science Advances, 7(42), eabh3995.

[2] DePalma, S. J., Davidson, C. D., Stis, A. E., Helms, A. S., & Baker, B. M. (2021). Microenvironmental determinants of organized iPSC-cardiomyocyte tissues on synthetic fibrous matrices. Biomaterials science, 9(1), 93-107.

In brief, standard devices of “Type 1” consist of 2 pillars of either circular or rectangular cross-sections with spherical caps. The devices were cast from poly(dimethylsiloxane) (PDMS) 3D printed molds and were then chemically treated to promote both cell attachment to the pillar caps and growth of the microbundle between the two pillars. Following sterilization of the device, hiPSC-CMs, differentiated and purified, were seeded with human ventricular cardiac fibroblasts and fibrin extracellular matrix (ECM) solution. Finally, time-lapse videos of tissue contractions, whether beating spontaneously or electrically stimulated with 30V at a 1Hz frequency, were taken 5–15 days after seeding.

The 732 examples included in this dataset were grown under 31 different tissue culture conditions, as detailed in the "Type1_experimental_conditions.pdf" document. The document "Type1_common_experimental_metadata.pdf" summarizes the common experimental conditions.

Usage notes

As previously mentioned, we include here 732 examples of "Type 1" tissue. We share the raw videos in ".tif" format and organize them based on: 1) platform type ("Type 1"), 2) experimental conditions under which the microbundles were grown ("condition_000", "condition_001",...), and 3) the batch of differentiated CMs ("batch_000", "batch_001",...) and is reflected in the folder naming.

Additionally, we include a metadata file ("metadata_type1.csv") that includes the "file_name", "experimental_conditions", "batch", "comments", and "included_in_plot". While "file_name", "experimental_conditions", and "batch" are straightforward to comprehend, "comments" correspond to remarks regarding running each specific example with MicroBundlePillarTrack software available on GitHub. The "included_in_plot" column is used to indicate if the example was included (marked by "1") or not (marked by "0") in the summary output plot included in the main figure of the accompanying manuscript. We note that the keys for the number-coded entries in "experimental_conditions" and "comments" are included in the "Type1_experimental_conditions.pdf" and "Comments_key.pdf" files.