Data from: PCSK1N as a tumor size marker and an ER stress response protein in corticotroph pituitary adenomas
Data files
Oct 01, 2024 version files 247.40 KB
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README.md
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Supplementary_file__-_DEPs_17AAG.csv
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Abstract
Purpose: Silent corticotroph adenoma (SCA) exhibits more tumor aggressiveness features than functioning adenomas (FCA). We aimed to investigate PCSK1N expression in CA and examine if ER stress-induced responses affect cell survival in a corticotroph tumor cell model.
Methods: Clinical and imaging characteristics were recorded in 33 patients with FCA (20 women,11 macroadenomas) and 18 SCA (8 women, all macroadenomas). Gene expression of proopiomelanocortin (POMC), T-box transcription factor 19(TBX19)/TPIT, proprotein convertase subtilisin/kexin type 1(PCSK1)/PC1/3, and its inhibitor PCSK1N, was measured by RT-qPCR in adenoma tissue. Mouse pituitary corticotroph tumor (AtT-20) cells were treated with tanespimycin (17-AAG), a HSP90 chaperone inhibitor, to induce ER stress followed by gene and protein analyses.
Results: POMC, TPIT, and PCSK1 expression were higher, whereas PCSK1N was lower in FCA compared to SCA. PCSK1N correlated with POMC (rs= -0.514, p <0.001), TPIT (rs= -0.386, p = 0.005), PCSK1 (rs= -0.3691, p = 0.008), and tumor largest diameter (rs= 0.645, p <0.001), in all CA. Induction of ER stress by 17-AAG in AtT-20 cells led to a decrease of POMC but an increase of PCSK1N gene expression at 24h. Moreover, a downregulation of cell cycle, apoptosis, and senescence pathways, and alterations in cell adhesion and cytoskeleton were observed at the protein level.
Conclusions: PCSK1N is higher in SCA compared with FCA, and associated with corticotroph cell markers and tumor size. PCSK1N is likely to be part of the adaptive response to ER stress, potentially conferring a survival advantage to the corticotroph tumor cell in conjunction with other proteins.
README: Supplementary files for PCSK1N as a Tumor Size Marker and an ER Stress Response Protein in Corticotroph Pituitary Adenomas
https://doi.org/10.5061/dryad.stqjq2c9n
Description of the data and file structure
This supplementary files contain;
- Supplementary file for human and mouse primers in Microsoft Word file
- Supplementary figure for DEPs heatmap control vs 17-AAG treatment in TIFF file
- Supplementary data for DEPs control vs 17-AAG treatment in csv file, containing;
- Relative quantification of peptides (LFQ intensity) - 7 controls (DMSO) and 4 17-AAG slots
- Student's T-test p-value 17AAG_DMSO
- Student's T-test q-value 17AAG_DMSO
- Student's T-test Difference 17AAG_DMSO - fold of change
- Protein IDs
- Protein names
- Gene names