Data from: Sex-specific effects of dietary restriction on physiological variables in Japanese quails
Data files
May 03, 2024 version files 246.05 KB
-
dataset_and_R_code.zip
-
README.md
Abstract
Nutritional limitation is a common phenomenon in nature that leads to trade-offs among processes competing for limited resources. These trade-offs are mediated by changes in physiological traits such as growth factors and circulating lipids. However, studies addressing the sex-specific effect of nutritional deficiency on these physiological variables are limited in birds. We used dietary restriction to mimic the depletion of resources to various degrees and investigated sex-specific effects on circulating levels of insulin-like growth factor 1 (IGF-1) and triglycerides in Japanese quails (Coturnix japonica) subjected to ad libitum, 20%, 30% or 40% restriction of their daily requirement, for two weeks. We also explored the association of both physiological variables with body mass and egg production. While dietary restriction showed no effects on circulating IGF-1, this hormone exhibited a marked sexual difference, with females having 64.7% higher IGF-1 levels than males. Dietary restriction significantly reduced plasma triglyceride levels in both sexes. Females showed more than six-fold higher triglyceride levels than males. Triglyceride levels were positively associated with body mass in females while showed not association in males. Overall, our findings revealed sex-specific expression of physiological variables under dietary restriction conditions, which coincide with body size.
README: Data from: Sex-specific effects of dietary restriction on physiological variables in Japanese quails
https://doi.org/10.5061/dryad.stqjq2cb0
The dataset includes plasma levels of insulin like growth factor 1 (IGF-1) and triglycerides from a dietary restriction experiment in Japanese quails. Quails aged nine weeks were subjected to either ad libitum feeding or 20%, 30%, and 40% restrictions for 14 days. Plasma samples were collected on day zero (initial), day seven (week 1), and day forteen (week 2) of the experiment period. Plasma levels of IGF-1 were measured using a competitive Enzyme-linked immunosorbent assay (ELISA), while triglyceride levels were determined using a photometric method.
Description of the data and file structure
1. Title: Data from: Sex-specific effects of dietary restriction on physiological variables in Japanese quails
2. Author Information
A. Principal Investigator (Contact Information)
Name: Gebrehaweria K. Reda
Institution: University of Debrecen
Address: Department of Animal Science, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary
Department of Evolutionary Zoology and Human Biology, Faculty of Life Science, University of Debrecen, 4032 Debrecen, Hungary
Email: gebrek2000@gmail.com or gebrek2000@agr.unideb.hu
B. Co-investigator 1
Name: Sawadi F. Ndunguru
Institution: University of Debrecen
Address: Department of Animal Science, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary
: Department of Evolutionary Zoology and Human Biology, Faculty of Life Science, University of Debrecen, 4032 Debrecen, Hungary
C. Co-investigator 2
Name: Brigitta Csernus
Institution: University of Debrecen
Address: Department of Evolutionary Zoology and Human Biology, Faculty of Life Science, University of Debrecen, 4032 Debrecen, Hungary
D. Co-investigator 3
Name: James K. Lugata
Institution: University of Debrecen
Address: Department of Animal Nutrition and Physiology, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary
E. Co-investigator 4
Name: Renáta Knop
Institution: University of Debrecen
Address: Department of Animal Science, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary
F. Co-investigator 5
Name: Csaba Szabó
Institution: University of Debrecen
Address: Department of Animal Nutrition and Physiology, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary
G. Supervisor
Name: Levente Czeglédi
Institution: University of Debrecen
Address: Department of Animal Science, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, Hungary
Email: czegledi@agr.unideb.hu
H. Supervisor
Name:Ádám Z. Lendvai
Institution: University of Debrecen
Address: Department of Evolutionary Zoology and Human Biology, Faculty of Life Science, University of Debrecen, 4032 Debrecen, Hungary
Email: azlendvai@gmail.com
3. Data collected: 2021
4. Geographic location of data collection: Institute of Agricultural Research and Educational Farm of the University of Debrecen, Debrecen, Hungary.
5. Founding source: National Research, Development and Innovation Office (OTKA K139021)
ACCESS INFORMATION
Recommended citation for this dataset: Reda et al. (2024), Data from: Sex-specific effects of dietary restriction on physiological variables in Japanese quails.
DATA & FILE OVERVIEW
File List:
File 1 Name: Data from: Sex-specific effects of dietary restriction on physiological variables in Japanese quails
File 1 Description: The effect different dietary restriction levels on plasma levels of IGF-1 and triglycerides in adult Japanese quails (Coturnix japonica).
METHODOLOGICAL INFORMATION
We used dietary restriction to mimic resource depletion and examined its effects on IGF-1 (ng/mL) and triglycerides (mmol/L) in Japanese quails. Quails were subjected to ad libitum feeding or 20%, 30%, and 40% restriction levels for 14 days. We colleced plasma samples and measured IGF-1 and triglycerides levels at the beginning of the experiment (day 0), on days 7 and on day 14 of the restriction period. We measured plasma IGF-1 levels using a competitive Enzyme-linked immunosorbent assay (ELISA) (https://doi.org/10.1007/s00265-020-2821-6. We mesured plasma triglyceride levels using a photometric method with a half-automatic analyser (Lab-Analyse, Orvostechnika Ltd., Budapest, Hungary).
DATA-SPECIFIC INFORMATION
1. Number of variables/columns: 10
2. Number of rows: 193
3. Variable List:
birdID
sex
block
stagger
session
handlingtime
treatment
week
igf1
trig
4. Missing data codes: na
5. Abbreviations used:
birdID; bird identification number.
sex; sex of birds
block; experimental block
stagger; numbers showing staggering batch
session; sample collection session in one day sampling
handlingtime; time from first aproach to the bird until final blood collection from that bird
treatment; different dietary restriction levels.
week; sampling and measurement time points
igf1; circulating level of IGF-1 in ng/mL measured using ELISA
trig; circulating level of triglycerides mmol/L, measured using photometric method
ADL; ad libitum.
DR20; 20% restricted.
DR30; 30% restricted.
DR40; 40% restricted.
Sharing/Access information
This is a section for linking to other ways to access the data, and for linking to sources the data is derived from, if any.
Links to other publicly accessible locations of the data:
- This data was used for a manuscript in progress entitled "Sex-specific effects of dietary restriction on physiological variables in Japanese quails". The mansucript is accepted for publication in Ecology and evolution journal.
Data was derived from the following sources:
Sex-specific effects of dietary restriction on physiological variables in Japanese quails
Code/Software
We used R v. 4.2.2 “Innocent and Trusting” software to statistically analyse and visualise our data. All the codes and the packeges used are described below.
Methods
We used dietary restriction to mimic resource depletion and examined its effects on IGF-1 (ng/mL) and triglycerides (mmol/L) in Japanese quails. Quails were subjected to ad libitum feeding or 20%, 30%, and 40% restriction levels for 14 days. We colleced plasma samples and measured IGF-1 and triglycerides levels at the beginning of the experiment (day 0), on days 7 and on day 14 of the restriction period. We measured plasma IGF-1 levels using a competitive IGF-1 Enzyme-linked immunosorbent assay (ELISA) (https://doi.org/10.1007/s00265-020-2821-6). We mesured plasma triglyceride levels using a photometric method with a half-automatic analyser (Lab-Analyse, Orvostechnika Ltd., Budapest, Hungary).