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Reproductive fitness of Callosobruchus maculatus mtDNA haplotypes

Citation

Immonen, Elina et al. (2021), Reproductive fitness of Callosobruchus maculatus mtDNA haplotypes, Dryad, Dataset, https://doi.org/10.5061/dryad.sxksn0319

Abstract

Mitochondrial DNA (mtDNA) consists of few but vital maternally inherited genes that interact closely with nuclear genes to produce cellular energy. How important mtDNA polymorphism is for adaptation is still unclear. The assumption in population genetic studies is often that segregating mtDNA variation is selectively neutral. This contrasts with empirical observations of mtDNA haplotypes affecting fitness related traits and thermal sensitivity, and latitudinal clines in mtDNA haplotype frequencies. Here we experimentally test whether ambient temperature affects selection on mtDNA variation, and whether such thermal effects are influenced by intergenomic epistasis due to interactions between mitochondrial and nuclear genes, using replicated experimental evolution in Callosobruchus maculatus seed beetle populations seeded with a mixture of different mtDNA haplotypes. We also test for sex-specific consequences of mtDNA evolution on reproductive success, given that mtDNA mutations can have sexually antagonistic fitness effects. Our results demonstrate natural selection on mtDNA haplotypes, with some support for thermal environment influencing mtDNA evolution through mitonuclear epistasis. The changes in male and female reproductive fitness were both aligned with changes in mtDNA haplotype frequencies, suggesting that natural selection on mtDNA is sexually concordant in stressful thermal environments. We discuss the implications of our findings for the evolution of mtDNA. 

Methods

See methods in our publication: Immonen et al. "An experimental test of temperature-dependent selection on mitochondrial haplotypes in Callosobruchus maculatus seed beetles". Ecology and Evolution (in print).

Usage Notes

This dataset contains fitness data (lifetime fecundity, tot_eggs) for three populations (nuc, levels = bra, ca or yem) that either contain a single mtDA haplotype (type 'pure', mito levels = BRA, CA or YEM) expressed in their native nuclear genetic background, or a mix of all three haplotypes (type 'mix). Each line contains a biological (bio_rep) and a technical replicate (tech_rep). The assays were repeated at genrations 3 and 33.