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Data from: Nutritional challenges of feeding a mutualist: testing for a nutrient-toxin tradeoff in fungus-farming leafcutter ants

Citation

Crumière, Antonin et al. (2022), Data from: Nutritional challenges of feeding a mutualist: testing for a nutrient-toxin tradeoff in fungus-farming leafcutter ants, Dryad, Dataset, https://doi.org/10.5061/dryad.sxksn0347

Abstract

The biochemical heterogeneity of food items often yields tradeoffs as each bite of food tends to contain some nutrients in surplus and others in deficit, as well as other less palatable or even toxic compounds. These multidimensional nutritional challenges are likely compounded when foraged foods are used to provision others (e.g. offspring or symbionts) with different physiological needs and tolerances. We explored these challenges in free-ranging colonies of leafcutter ants that navigate a diverse tropical forest to collect plant fragments they use to provision a co-evolved fungal cultivar. We tested the prediction that leafcutter farmers face provisioning tradeoffs between the nutritional quality and concentration of toxic tannins in foraged plant fragments. Chemical analyses of plant fragments sampled from the mandibles of Panamanian Atta colombica leafcutter ants provided little support for a nutrient-tannin foraging tradeoff. First, colonies foraged for plant fragments ranging widely in tannin concentration. Second, high tannin levels did not appear to restrict colonies from selecting plant fragments with blends of protein and carbohydrates that maximized cultivar performance when measured with in vitro experiments. We also tested whether tannins expand the realized nutritional niche selected by leafcutter ants into high-protein dimensions since: 1) tannins can bind proteins and reduce their accessibility during digestion, and 2) in vitro experiments have shown that excess protein provisioning reduces cultivar performance. Contrary to this hypothesis, the most protein-rich plant fragments did not have highest tannin levels. More generally, the approach developed here can be used to test how multidimensional interactions between nutrients and toxins shape the costs and benefits of providing care to offspring or symbionts.

Methods

Dataset contains: 1) data from fungus performance obtained with in vitro fungal culture in the lab, 2) published quantification of protein and carbohydrate in plant samples (reference explicitly cited in the manuscript), and 3) tannin quantification from plant samples acquried after fieldwork collection.

Usage Notes

Please consult the ReadMe files for description of variables and data.

Funding

European Research Council, Award: ERC-2017-STG-757810