To estimate the influence of developmental temperature and Wolbachia infections on body size in Drosophila melanogaster, we disected female flies and mounted left forelegs. Pictures were taken under a Leica DFC 490 microscope. Femur lengths, wich have proven to be a good proxy for bodysize, were measured in ImageJ. Our analyses revealed that temperature, but not Wolbachia infections strongly influenced female body size. See also the corresponing README.txt and the table "FullData.xlsx" within SupplementaryData.zip in the supplement of the corresponding manuscript "Complex effects of environment and Wolbachia infections on the life history of Drosophila melanogaster hosts" (DOI: 10.1111/jeb.14016) for more details.