Data from: Accumulation curves of environmental DNA sequences predict coastal fish diversity in the Coral Triangle
Cite this dataset
Juhel, Jean-Baptiste et al. (2020). Data from: Accumulation curves of environmental DNA sequences predict coastal fish diversity in the Coral Triangle [Dataset]. Dryad. https://doi.org/10.5061/dryad.t1g1jwt05
Abstract
Methods
Environmental DNA (eDNA) was extracted from 2L seawater samples as described in the file Methods section of the paper. Retrieved eDNA was then amplified using a teleost-specific 12S mitochondrial rDNA primer (teleo), sequenced and processed using two bioinformatics pipelines. The files obitools_lengguru.csv and swarm_lengguru.csv are the outputs of the OBITools and SWARM pipeline, respectively. It represents the raw data merged with metadata and linking the occurence tables with taxonomical assignments, prior to quality filtering.
Usage notes
The bioinformatic pipelines scripts used to generate both datasets are available in Gitlab following the links below :
https://gitlab.mbb.univ-montp2.fr/edna/snakemake_only_obitools
https://gitlab.mbb.univ-montp2.fr/edna/bash_swarm