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Single molecule images for: An empirical energy landscape reveals mechanism of proteasome in polypeptide translocation

Citation

Lu, Ying (2021), Single molecule images for: An empirical energy landscape reveals mechanism of proteasome in polypeptide translocation, Dryad, Dataset, https://doi.org/10.5061/dryad.t1g1jwt2t

Abstract

The ring-like ATPase complexes in the AAA+ family perform diverse cellular functions that require coordination between the conformational transitions of their individual ATPase subunits.  How the energy from ATP hydrolysis is captured to perform mechanical work by these coordinated movements is not known.  In this study, we developed a novel approach for delineating the nucleotide-dependent free-energy landscape (FEL) of the proteasome's heterohexameric ATPase complex based on complementary structural and kinetic measurements.  We used the FEL to simulate the dynamics of the proteasome and quantitatively evaluated the predicted structural and kinetic properties. The FEL model predictions were widely consistent with experimental observations in this and previous studies and suggested novel features of the mechanism of proteasomal ATPase. We find that the cooperative movements of the ATPase subunits result from the design of the ATPase hexamer entailing a unique free-energy minimum for each nucleotide-binding state.  ATP hydrolysis dictates the direction of substrate translocation by triggering an energy-dissipating conformational transition of the ATPase complex.

Funding

National Institutes of Health, Award: GM134064-01

Edward Mallinckrodt, Jr. Foundation, Award: N/A