Data from: Reliable, verifiable and efficient monitoring of biodiversity via metabarcoding
Data files
Aug 05, 2013 version files 214.87 MB
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Ailaoshan_all_Map.txt
3.37 KB
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Ailaoshan.1-4.TCA.454Reads.fna.qual.zip
66.54 MB
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arthropods.env.txt
2.84 KB
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Danum_Valley.1.2.TCA.454Reads.fna.qual.zip
126.64 MB
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Example_R_script.R
12.06 KB
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LahadDatu_all_Map.txt
2.68 KB
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MBCarthropods.txt
41.53 KB
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README_for_Ailaoshan_all_Map.txt
3.11 KB
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README_for_LahadDatu_all_Map.txt
3.11 KB
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README_for_UK1_all_Map.txt
3.11 KB
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RSW2_Test.R
5.78 KB
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RSW2SoftwareResults.txt
788 B
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UK1_all_Map.txt
6.86 KB
Jul 10, 2015 version files 363.17 MB
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Ailaoshan_all_Map.txt
3.37 KB
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Ailaoshan.1-4.TCA.454Reads.fna.qual.zip
66.54 MB
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arthropods.env.txt
2.84 KB
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arthropods.txt
29.31 KB
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Danum_Valley.1.2.TCA.454Reads.fna.qual.zip
126.64 MB
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Example_R_script.R
12.06 KB
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LahadDatu_all_Map.txt
2.68 KB
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MBCarthropods.txt
41.53 KB
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README_for_Ailaoshan_all_Map.txt
3.11 KB
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README_for_LahadDatu_all_Map.txt
3.11 KB
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README_for_UK1_all_Map.txt
3.11 KB
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RSW2_Test.R
5.78 KB
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RSW2SoftwareResults.txt
788 B
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Thetford.1.2.3.10.TCA.454Reads.fna.qual.zip
21.60 MB
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UK1_all_Map.txt
6.86 KB
Abstract
To manage and conserve biodiversity, one must know what is being lost, where, and why, as well as which remedies are likely to be most effective. Metabarcoding technology can characterise the species compositions of mass samples of eukaryotes or of environmental DNA. Here, we validate metabarcoding by testing it against three high-quality standard data sets that were collected in Malaysia (tropical), China (subtropical) and the United Kingdom (temperate) and that comprised 55,813 arthropod and bird specimens identified to species level with the expenditure of 2,505 person-hours of taxonomic expertise. The metabarcode and standard data sets exhibit statistically correlated alpha- and beta-diversities, and the two data sets produce similar policy conclusions for two conservation applications: restoration ecology and systematic conservation planning. Compared with standard biodiversity data sets, metabarcoded samples are taxonomically more comprehensive, many times quicker to produce, less reliant on taxonomic expertise and auditable by third parties, which is essential for dispute resolution.