Something is not quite right: effects of two land uses on anuran diversity in subtropical grasslands
Moreira, Leonardo Felipe Bairos; Castilhos, Henrique Zanette de; Castroviejo-Fisher, Santiago (2020), Something is not quite right: effects of two land uses on anuran diversity in subtropical grasslands, Dryad, Dataset, https://doi.org/10.5061/dryad.tdz08kpx4
In the spring of 2015 (10–16 October), we sampled tadpoles in 22 ponds located 5.5–316 km apart in southern Brazil. In order to ensure that the landscape composition surrounding each pond did not change over different scales, we defined circular areas (1,000 m radius) and measured land-cover data , using Qgis 2.18.16 . We based analysis on Google Earth imagery, using a land-cover classification for 2015 produced within the scope of the MapBiomas initiative. At each pond, we performed eight dip-net sweeps (30 cm diameter, 2mm mesh), each covering approximately 1 m2 and distributed at different pond depths (i.e., four sweeps near the edge of the pond and four sweeps in deep water near the centre of the pond). Sweeps were pooled into one sample per pond (3.5 L plastic bucket), where the collected tadpoles were euthanized with a benzocaine solution and sorted into series based on their morphology (i.e. coloration, body shape, eyes position, tail fin proportion, and mouth position). We also registered the presence of all observed adults, visually or acoustically, during tadpole sampling. In laboratory, each tadpole without tail clip was rinsed in distilled water and then measured for a set of eight morphological traits on an automated stereomicroscope – Leica M205A.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Award: 20132816
Conselho Nacional de Desenvolvimento Científico e Tecnológico, Award: 442987/20