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Data from: Comparison of taxon-specific versus general locus sets for targeted sequence capture for plant phylogenomics

Citation

Chau, John H.; Rahfeldt, Wolfgang A.; Olmstead, Richard G. (2019), Data from: Comparison of taxon-specific versus general locus sets for targeted sequence capture for plant phylogenomics, Dryad, Dataset, https://doi.org/10.5061/dryad.v6q0p

Abstract

Premise of the study: Targeted sequence capture can be used to efficiently gather sequence data for large numbers of loci, such as single-copy nuclear loci. Most published studies in plants have used taxon-specific locus sets developed individually for a clade using multiple genomic and transcriptomic resources. General locus sets can also be developed from loci that have been identified as single-copy and having orthologs in large clades of plants. Methods: We identify and compare a taxon-specific locus set and three general locus sets (COSII, APVO SSC, PPR) for targeted sequence capture in Buddleja (Scrophulariaceae) and outgroups. We evaluate their performance in terms of assembly success, sequence variability, and resolution and support of inferred phylogenetic trees. Results: The taxon-specific locus set had the most target loci. Assembly success was high for all locus sets in Buddleja samples. For outgroups, general locus sets had greater assembly success. Taxon-specific and PPR loci had the highest average variability. The taxon-specific dataset produced the best supported tree, but all datasets showed improved resolution over previous non-sequence capture datasets. Discussion: General loci can be a useful source of sequence capture targets, especially if multiple genomic resources are not available for a taxon.

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Funding

National Science Foundation, Award: DEB-1020369, DEB-1311111