Differences in thermal tolerance between parental species could fuel thermal adaptation in hybrid wood ants
Martin-Roy, Raphael; Nygård, Elisa; Nouhaud, Pierre; Kulmuni, Jonna (2021), Differences in thermal tolerance between parental species could fuel thermal adaptation in hybrid wood ants, Dryad, Dataset, https://doi.org/10.5061/dryad.v6wwpzgvh
The purification of total DNA was performed with DNeasy Tissue kits (Qiagen) following the manufacturer’s spin-column protocol for insects. Samples were genotyped based on previous genetic studies using nine microsatellite markers (Fe7, Fe17, Fy3, Fe19, Fe13, Fy15, Fy12, Fl29, and Fy13) and PCR conditions determined in earlier studies (Kulmuni et al., 2010; Beresford et al., 2017). Total DNA was amplified with polymerase chain reactions (PCR) in Veriti 96-well Thermal Cycler (Applied Biosystems) with fluorescent labelling. Genotypes were resolved by capillary electrophoresis with a 3730 DNA Analyzer (Applied Biosystems) using 500 ROX size standard. Lastly, genotypes were scored with GENEMAPPER version 4.0 (Applied Biosystems). As genotypes were compared to samples genotyped in an earlier study on the same system (Beresford et al., 2017) we used three reference samples of known genotypes in every PCR reaction to control for variation potentially introduced by different PCR and DNA Analyzer machines.