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Agriculture creates subtle genetic structure among migratory and non-migratory populations of burrowing owls throughout North America

Citation

Macías-Duarte, Alberto; Conway, Courtney; Culver, Melanie (2021), Agriculture creates subtle genetic structure among migratory and non-migratory populations of burrowing owls throughout North America, Dryad, Dataset, https://doi.org/10.5061/dryad.vdncjsxrx

Abstract

Population structure across a species distribution primarily reflects historical, ecological and evolutionary processes. However, large-scale contemporaneous changes in land use have the potential to create changes in habitat quality and thereby cause changes in gene flow, population structure, and distributions. As such, land-use changes in one portion of a species range may explain declines in other portions of their range. For example, many burrowing owl populations have declined or become extirpated near the northern edge of the species’ breeding distribution during the second half of the 20th century. In the same period, large extensions of thornscrub were converted to irrigated agriculture in northwestern Mexico. These irrigated areas may now support the highest densities of burrowing owls in North America. We tested the hypothesis that burrowing owls that colonized this recently created owl habitat in northwestern Mexico originated from declining migratory populations from the northern portion of the species’ range (migration-driven breeding dispersal whereby long-distance migrants from Canada and the U.S. became year-round residents in the newly created irrigated agriculture areas in Mexico). We used 10 novel microsatellite markers to genotype 1,560 owls from 36 study locations in Canada, Mexico, and the United States. We found that burrowing owl populations are practically panmictic throughout the entire North American breeding range. However, an analysis of molecular variance provided some evidence that burrowing owl populations in northwestern Mexico and Canada together are more genetically differentiated from the rest of the populations in the breeding range, lending some support to our migration-driven breeding dispersal hypothesis. We found evidence of subtle genetic differentiation associated with irrigated agricultural areas in southern Sonora and Sinaloa in northwestern Mexico. Our results suggest that land-use can produce location-specific population dynamics leading to subtle genetic structure even in the absence of dispersal barriers.

Methods

We obtained DNA samples from 1,560 breeding burrowing owls from 36 locations in Canada, Mexico, and the United States. We trapped burrowing owls during the summers of 2004-2009. None of the 1,560 birds that we included in our analysis were closely related (i.e., a parent and its offspring, or >1 juvenile from the same nest burrow). Our primary source of genomic DNA was blood. We obtained ~50 μL of blood through a venipuncture of the brachial vein. We also used flight and/or body feathers occasionally as a source of genomic DNA when we could not withdraw a blood sample. We used 10 microsatellite markers developed specifically for this study (Macías-Duarte, Conway, Vega-Munguía, & Culver, 2010) to obtain genotypic data from our 36 study locations. We analyzed PCR products on an Applied Biosystems 3730 Genetic Analyzer and used an Applied Biosystems Genotyper 3.7 to score alleles. We used program Tandem (Matschiner & Salzburger, 2009) to assign integers to DNA fragment sizes.

Usage Notes

This dataset contains the phenotypes of each of 1,560 burrowing owl. Missing data is coded as 0.