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Data and source code from: Contingency and selection in mitochondrial genome dynamics

Citation

Nunn, Christopher; Goyal, Sidhartha (2022), Data and source code from: Contingency and selection in mitochondrial genome dynamics , Dryad, Dataset, https://doi.org/10.5061/dryad.vdncjsxwx

Abstract

Eukaryotic cells contain numerous copies of mitochondrial DNA (mtDNA), allowing for the coexistence of mutant and wild-type mtDNA in individual cells. The fate of mutant mtDNA depends on their relative replicative fitness within cells and the resulting cellular fitness within populations of cells. Yet the dynamics of the generation of mutant mtDNA and features that inform their fitness remain unaddressed. Here we utilize long read single-molecule sequencing to track mtDNA mutational trajectories in Saccharomyces cerevisiae. We show a previously unseen pattern that constrains subsequent excision events in mtDNA fragmentation. We also provide evidence for the generation of rare and contentious non-periodic mtDNA structures that lead to persistent diversity within individual cells. Finally, we show that measurements of relative fitness of mtDNA fit a phenomenological model that highlights important biophysical parameters governing mtDNA fitness. Altogether, our study provides techniques and insights into the dynamics of large structural changes in genomes that may be applicable in more complex organisms.

Methods

Dataset was collected with an Oxford Nanopore MinION Mk1B following the experimental description in methods. Raw Nanopore reads were basecalled and demultiplexed with the ONT Guppy package 3.1.5-1.

Usage Notes

See README file

Funding