Data from: Expression plasticity and evolutionary changes extensively shape the sugar-mimic alkaloid adaptation of non-digestive glucosidase in lepidopteran mulberry-specialist insects
Li, Xiaotong, Zhejiang University
Shi, Liangen, Zhejiang University
Dai, Xiangping, Zhejiang University
Chen, Yajie, Zhejiang University
Xie, Hongqing, Zhejiang University
Feng, Min, Zhejiang University
Chen, Yuyin, Zhejiang University
Wang, Huabing, Zhejiang University
Published May 17, 2018 on Dryad.
Cite this dataset
Li, Xiaotong et al. (2018). Data from: Expression plasticity and evolutionary changes extensively shape the sugar-mimic alkaloid adaptation of non-digestive glucosidase in lepidopteran mulberry-specialist insects [Dataset]. Dryad. https://doi.org/10.5061/dryad.vh7kh99
During the co-evolutionary arms race between plants and herbivores, insects evolved systematic adaptive plasticity to minimise the chemical defence effects of their host plants. Previous studies mainly focused on the expressional plasticity of enzymes in detoxification and digestion. However, the expressional response and adaptive evolution of other fundamental regulators against host phytochemicals are largely unknown. Glucosidase II (GII), which is composed of a catalytic GIIα subunit and a regulatory GIIβ subunit, is an evolutionarily conserved enzyme that regulates glycoprotein folding. In this study, we found that GIIα expression of the mulberry-specialist insect was significantly induced by mulberry leaf extract, 1-Deoxynojirimycin (1-DNJ), whereas GIIβ transcripts were not significantly changed. Moreover, positive selection was detected in GIIα when the mulberry-specialist insects diverged from the lepidopteran order; whereas GIIβ was mainly subjected to purifying selection, thus indicating an asymmetrically selective pressure of GII subunits. In addition, positively selected sites were enriched in the GIIα of mulberry-specialist insects, and located around the 1-DNJ binding sites and in the C-terminal region, which could result in conformational changes that affect catalytic activity and substrate-binding efficiency. These results show that expression plasticity and evolutionary changes extensively shape sugar-mimic alkaloids adaptation of non-digestive glucosidase in lepidopteran mulberry-specialist insects. Our study provides novel insights into a deep understanding of the sequestration and adaptation of phytophagous specialists to host defensive compounds.
Raw qRT-PCR data
The data file provides the raw Ct values for the qRT-PCR assay. The qRT-PCR assay was performed using total RNA isolated from midgut of 4th instar larvae of B. mori or S. c. ricini. Three biological replicates and three technical replicates were performed for each sample. The coding gene of Ribosomal protein 49 of B. mori and S. c. ricini was used as an internal control.