Heatwave grazing kelp microbes sequences
Data files
Sep 15, 2023 version files 1.89 GB
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README.md
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Abstract
The range-expansion of tropical herbivores due to ocean warming can profoundly alter temperate reef communities by overgrazing the seaweed forests that underpin them. Such ecological interactions may be mediated by changes to seaweed-associated microbiota in response to warming, but empirical evidence demonstrating this is rare. We experimentally simulated ocean warming and marine heatwaves (MHWs) to quantify effects on two dominant temperate seaweed species and their microbiota, as well as grazing by a tropical herbivore. The kelp Ecklonia radiata’s microbiotain sustained warming and MHW treatments were enriched with microorganisms associated with seaweed disease and tissue degradation. In contrast, the fucoid Sargassum linearifolium’s microbiota was unaffected by temperature. Consumption by the tropical sea-urchin Tripneustes gratilla was greater on Ecklonia where the microbiota had been altered by higher temperatures, while Sargassum’s consumption was unaffected. Elemental traits (carbon, nitrogen), chemical defences (phenolics) and tissue bleaching of both seaweeds were generally unaffected by temperature. Effects of warming and MHWs on seaweed holobionts (host plus its microbiota) are likely species-specific. The effect of increased temperature on Ecklonia’s microbiota and subsequent increased consumption suggest that changes to kelp microbiota may underpin kelp-herbivore interactions, providing novel insights into potential mechanisms driving change in species’ interactions in warming oceans.
README: Heatwave grazing kelp microbes sequences
https://doi.org/10.5061/dryad.vhhmgqns7
We experimentally simulated ocean warming and marine heatwaves (MHWs) to quantify effects on two dominant temperate seaweed species and their microbiota, as well as grazing by a tropical herbivore. The kelp Ecklonia radiata’s microbiota in sustained warming and MHW treatments were enriched with microorganisms associated with seaweed disease and tissue degradation. In contrast, the fucoid Sargassum linearifolium’s microbiota was unaffected by temperature*.* Consumption by the tropical sea-urchin Tripneustes gratilla was greater on Ecklonia where the microbiota had been altered by higher temperatures, while Sargassum’s consumption was unaffected. Elemental traits (carbon, nitrogen), chemical defences (phenolics) and tissue bleaching of both seaweeds were generally unaffected by temperature.
Description of the data and file structure
Juvenile Ecklonia radiata (length ~15cm; N=140) and Sargassum linearifolium (length ~10cm; N=140) were collected haphazardly (>2m apart) at Cronulla rocky reef, Sydney, Australia. We exposed seaweeds to one of four temperature profiles over seven weeks: Ambient, Warming, marine heatwave MHW, MHW variable. After seven weeks of exposure to temperature treatments, a subset of individuals from each species/temperature treatment (Ecklonia: n=4-6; Sargassum: n=3) were randomly selected. Sterile cotton swabs were used to sample microbiota on algal surfaces, with the same area (20cm2) and swabbing time (30s) sampled for all individuals. Swabs were immediately stored in liquid nitrogen and transported to the University of New South Wales (UNSW, Sydney) and kept at -80°C until DNA extraction.
DNA was extracted from swabs using the DNeasy PowerSoil Kit (Qiagen) and amplified using Polymerase Chain Reaction (PCR) primers 341F (5’-CCTACGGGNGGCWGCAG-3’) and 785R (5’-GACTACHVGGGTATCTAATCC-3’), targeting the 16S rRNA gene V3-V4 regions (bacteria and archaea), and were sequenced with a 2x250bp MiSeq reagent kit v2 on the Illumina MiSeq2000 Platform.