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Data from: Systematics of Mukdenia and Oresitrophe (Saxifragaceae): Insights from genome skimming data

Chen, Mengzhen1; Deng, Pan1; Liu, Luxian1

Published Dec 28, 2021; Updated Jan 08, 2022 on Dryad. https://doi.org/10.5061/dryad.vq83bk3tq

Data files

Dec 28, 2021 version files 330.37 KB
Jan 08, 2022 version files 2.81 MB
Jan 08, 2022 version files 2.81 MB

Abstract

Oresitrophe and Mukdenia (Saxifragaceae) are epilithic sister genera used in traditional Chinese medicine. However, the taxonomy of Mukdenia, especially of M. acanthifolia, has been controversial. Genome skimming of M. acanthifolia and M. rossii, including three individuals for each species is reported and complete plastomes, partial mitogenomes and ETS/ITS sequences were assembled using these data. Their plastomes ranged from 156,341 bp to 157,018 bp in length and had similar structural characteristics and gene content of other flowering plants. These genomes were typically quadripartite, comprising 113 unique genes (79 protein coding genes, 30 tRNAs and 4 rRNAs). Comparative analysis showed that the plastomes of Mukdenia and Oresitrophe were relatively conservative, reflecting the genome size and structure, gene contents, RNA editing sites and codon usage. Five plastid regions that were hotspots of change (trnH-psbA, psbC-trnS, trnM-atpE, petA-psbJ and ccsA-ndhD) were identified within Mukdenia, and six regions (trnH-psbA, petN-psbM, trnM-atpE, rps16-trnQ, ycf1 and ndhF) contained a higher number of species-specific parsimony-informative sites that can serve as potential DNA barcodes for species identification. To infer phylogenetic relationships between Mukdenia and Oresitrophe, we combined our data with published data based on three different datasets. The monophyly of each species (O. rupifraga, M. acanthifolia and M. rossii) and the inferred topology ((M. rossii, M. acanthifolia), O. rupifraga) were well supported in trees reconstructed using the complete plastomes, but M. acanthifolia and M. rossii did not form separate clade in the ETS+ITS data. We found low recovery of genes in the Angiosperms 353 target enrichment panel from our unenriched genome skimming data. Hybridization or incomplete lineage sorting may be the cause of discordances between the trees reconstructed from plastid and nuclear data. The results from this study suggest that M. acanthifolia should be treated as a distinct species.