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Row data from: Screening and response profile of Iraqi barley accessions under PEG- induced drought conditions

Citation

Tahir, Nawroz (2020), Row data from: Screening and response profile of Iraqi barley accessions under PEG- induced drought conditions, Dryad, Dataset, https://doi.org/10.5061/dryad.vx0k6djqc

Abstract

Among the abiotic stresses, drought influences plant growth and productivity, and plant stress responses depend on stress intensity and genotype. The effects of simulated drought stress on germination parameters were tested for tolerance and sensitivity in 59 barley accessions from all regions of Iraq and phenotypic, physiological and biochemical responses associated with drought stress were investigated. Stress treatments with polyethylene glycol (PEG) reduced the germination rate and dramatically delayed the development of the seedlings. Significant changes in the physiological and biochemical profiles in barley accessions were observed. A smaller reduction in water absorption of seeds was observed across all accessions, while all biochemical characteristics except total flavonoid content were increased with the increase in water deficiency in most accessions. Among the 59 barley accessions, ABN was the most tolerant accession, while Black-Garmiyan was the most drought-prone. Significant relationships were found between most trait pairs, with 32 significant associations under no stress, 39 significant correlations under the 10.25% PEG treatment, and 81 significant correlations under the 20.50% PEG treatment. According to the key importance analysis, seed water uptake, total phenol content, soluble sugar content and catalase are classified as relative significance traits for germination percentage, root and shoot length under stress conditions.

Methods

All data were collected from an in vitro experiment. The data represent three replications of various phenotypic, physiological and biochemical characteristics obtained from barley seedlings.All data were collected from an in vitro experiment. The data represent three replications of various phenotypic, physiological and biochemical characteristics obtained from barley seedlings.

Full name of abbreviation:

Trait

Abbreviation

Unit

Germination percentage

GP

%

Root length

RL

cm

Shoot length

SL

cm

Seedling length

SEL

cm

Seed vigor

SV

-

Seed water uptake-40 h

WU-40

g

Seed water uptake-64 h

WU-64

g

Relative water content

RWC

%

Electrolyte leakage

EL

%

Total phenol content

TPC

µg GAE/gm FM

Total flavonoid content

TFC

µg QE/gm FM

Antioxidant assay (DPPH)

DPPH

µg Trolox/gm FM

Soluble sugar content

SSC

µg/g FW

Proline content

PC

µg/g FW

Guaiacol peroxidase activity

GPA

units/min/g FW

Catalase

CAT

units/min/g FW