Comparative seagulls of gut microbiota by using metagenomics and 16S rDNA sequencing
Data files
Sep 29, 2023 version files 353.18 KB
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README.md
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Table1.xlsx
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Table2.xlsx
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Abstract
Shotgun metagenomic and 16S rDNA sequencing are commonly used methods to identify the taxonomic composition of microbial communities. We compared the metagenome and 16S rDNA amplicon results to demonstrate the features of this animal. In general, relatively consistent patterns and reliability could be identified by both sequencing methods, but the results varied following the refinement of taxonomic levels. Metagenomic sequencing was more suitable for the discovery and detection of pathogenic bacteria of gut microbiota in seagulls. Although there were large differences in the numbers and abundance of bacterial species of the two methods in terms of taxonomic levels, the patterns and reliability results of the samples were consistent.
README: Comparative seagulls of gut microbiota by using metagenomics and 16S rDNA sequencing
https://doi.org/10.5061/dryad.w9ghx3fvw
We compared the metagenome and 16S rDNA amplicon results to identify the taxonomic composition of microbial communities.
Description of the data and file structure
Table1. The total taxa abundance of all samples in this study.
-sheet1. The total taxa abundance of all samples for metagenomics
-sheet2. The total taxa abundance of all samples for 16S rDNA sequencing
Table2. The original data of cladogram for common and unique taxa composition between the two methods.
Sharing/Access information
Data was derived from the following sources: The sequence data have been deposited into the National Center for Biotechnology Information (NCBI), https://www.ncbi.nlm.nih.gov/ with BioProject accession number: PRJNA849401 for metagenome and PRJNA849758 for 16S rDNA sequencing.
Code/Software
We have run the scripts files according to the request.