Diversity of cellular slime molds (dictyostelids) in the Fanjing Mountain Nature Reserve and geographical distribution comparisons with other representative nature reserves in different climate zones of China
Data files
Nov 06, 2023 version files 139.96 KB
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dataset_contents.zip
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README.md
Abstract
Protected areas are widely considered an essential strategy for biodiversity conservation. Dictyostelids are unique soil protists known to have important ecological functions, but the relationship between dictyostelid diversity within protected areas remains poorly understood, especially on a large scale. Herein, we report data on the distribution of dictyostelids, identified with ITS + SSU rRNA molecular and morphology-based taxonomy, from soil samples collected in the Fanjing Mountain protected area of Guizhou Province, Southwest China. We compared the dictyostelids biodiversity data with similar data from previously sampled sites in other protected areas in China. We identified four species of dictyostelids belonging to three genera (Dictyostelium, Heterostelium, and Polysphondylium)and herein provide information on the taxonomy of these species information. Two species (Heterostelium pallidum and Dictyostelium purpureum) are common and widely distributed throughout the world, but one species (Polysphondylium fuscans) was new to China. Our data indicate that the distribution patterns of dictyostelids are most influenced by vegetation and temperature. Overall, the similarity index between Baiyun Mountain in Henan Province and Fanjing Mountain in Guizhou Province, located at approximately the same longitude, is the highest, and the similarity coefficients of family, genus and species are 100%, 100% and 12.5%, respectively. From a species perspective, species in the same climate zone are not closely related, but obvious geographical distributions are evident in different climate zones. This preliminary study provided evidence of the ecological adaptation of dictyostelids to different biological niches.
README: Diversity of cellular slime molds (dictyostelids) in the Fanjing Mountain Nature Reserve and geographical distribution comparisons with other representative nature reserves in different climate zones of China
https://doi.org/10.5061/dryad.w9ghx3fw1
This study collected soil samples from Fanjing Mountain Nature Reserve, China, the nine-point sampling method with respect to three representative forest types (mixed forest, broadleaf forest, and coniferous forest) to isolate dictyodtelids. The brief summary of dataset contents:
1. Summary geographical data on the sampling
2. Dictyostelid species identification
3. Dictyostelid ecological data
4. Comparison and geographical distribution data of dictyostelids in representative natural reserves, China
Description of the data and file structure
1. Summary geographical data on the sampling (File 1)
[Soil nos., vegetation, coordinates, elevation (m), temperature, air humidity, pH, soil water moisture]
Soil nos. (6792–6980) recorded in the laboratory soil sample database; we used GPS real-time altitude (mobile application) to collect geographical data (i.e., vegetation, coordinates, elevation (m), temperature, air humidity) on the sampling in late August 2022 represented by plots numbered P1–P3 and the quadrats numbered A1–A3, B1–B3, C1–C3; the pH values of the soil dilutions were determined with a PHS-3C pH meter. Soil water moisture (%) was determined using a gravimetric analysis.
2. Dictyostelid species identification (File 2 and File 3)
[Taxonomy data (morphological observations and phylogenetic analysis)]
The characteristic stages in the life cycle, including cell aggregation and the formation of pseudoplasmodia and sorocarps, were observed under a Zeiss dissecting microscope (Axio Zoom V16) with a 1.5× objective and 10× ocular. Slides with sorocarps were prepared with water as the mounting medium. Features of spores, sorophores, and sorocarps were observed and measured on the slides by using a Zeiss light microscope (Axio Imager A2), with a 10× ocular and 10, 40, and 100× (oil) objectives. Photographs were obtained with a Zeiss Axiocam 506 color microscope camera.
The dictyostelid ITS and SSU rRNA sequencing data and obtained in the this study were uploaded to the NCBI database (accession numbers OR294048, OR294050, OR294051, OR294052, OR295629, OR295630, OR295628, OR295631).
3. Dictyostelid Ecological data (File 4)
[Clones Nos., density (clones/g), frequency (%) and relative density (%)] (Cavender and Raper 1965)
4. Comparison and geographical distribution data of dictyostelids in representative natural reserves, China (File 5)
The dictyostelids in the five protected areas: (dictyostelid species) Fig.13D
Fanjing Mountain in Guizhou Province (FJ) 4 species [in this study]
Changbai Mountain in Jilin Province (CB) 7 species [Zou et al. 2022]
Gushan Mountain in Fujian Province (GS) 3 species [Zhao et al. 2014]
Baiyun Mountain in Henan Province (BY) 5 species [Li et al. 2014]
Qinghai-Tibet Plateau in Tibet (QT) 12 species [Liu et al. 2019c]
[Jaccard similarity coefficient (SJ %) of the family, genus, and species]
SJ (%) = a × 100%/(a+b+c), where SJ is the similarity coefficient of the corresponding classification units in the two comparison areas, a is the number of shared classification units, b and c are the number of classification units that only appear in one place, respectively (Fig.13B)
We divided the dictyostelids in different provinces according to the climate zone Subtropical (6 species), Temperate (11 species), Plateau (12 species)
Sharing/Access information
Sequence links to publicly accessible locations of the data:
https://www.ncbi.nlm.nih.gov/
Code/Software
Dictyostelid Ecological data (File 4) processing in this study was completed in Excel, using the Hiplot online tool (https://hiplot.com.cn/home/index.html) to draw the diagram. The RDA (Redundancy analysis) including environmental factor (txt) and grouping (txt) (File 6) and Beta diversity (File 7) (PCA and clustered bar chart by Bray-Curtis similarities) using the cloud platform (http://www.cloud.biomicroclass.com/).
File 6 includes three parts: species abundance table (txt), environmental factor list file (txt), analysis type (txt)
[Species abundance table (txt)] The abundance table represents the number of species of dictyostelids isolated from different samples
[Environmental factor list file (txt)]
- pH
- Water moisture (%)
- Vegetation [Coniferous forest (1), Broadleaf forest (2), Mixed forest (3)]
- Elevation (m)
- Air humidity (%)
- Longitude(E)
- Latitude (N)
- Temperature (°C)
[analysis type (txt)] Group the samples