Nutribloods: Novel synthetic lepidopteran haemolymphs for understanding insect-microbe nutritional interactions in vitro
Data files
Jan 03, 2025 version files 171.46 KB
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dat10_archive.xlsx
43.34 KB
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dat2_archive.xlsx
126.96 KB
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README.md
1.16 KB
Abstract
Understanding the role of nutrients in microbial population dynamics relies on a sound appreciation of their nutritional environment and how this may vary in different habitats. For microbial pathogens and commensals, this can be especially challenging because the microbe may share nutritional resources with its host. Here we design a series of 20 synthetic cell-free haemolymphs (nutribloods) that mimic haemolymph nutrient profiles of caterpillars fed on one of 20 chemically-defined diets, that vary in their protein:carbohydrate (P:C) ratio and caloric density. Using these, we are able to simulate the range of nutritional conditions that insect blood pathogens might face, providing a model system for understanding the role of nutrition in microbial growth. We tested this using the entomopathogen, Xenorhabdus nematophila, a gram-negative extracellular bacterium of insect hosts. This revealed that whilst bacterial fitness peaked in nutriblood nutrient space that was high in carbohydrates and low in proteins, levels of amino acids in the nutribloods also appear to be an important driving force for bacterial growth. Using synthetic haemolymphs that had average levels of all nutrients other than carbohydrate, protein or amino acids, we also established that bacterial growth is generally enhanced by carbohydrate and amino acids but reduced by proteins. Here, we have established a tractable model system for examining the role that nutrition plays in the growth of an entomopathogenic bacterium. In future work, this model host-pathogen system can be used to test a range of nutritionally-driven processes, including competition during co-infection and interactions with the host microbiome, as well as comparative studies of other entomopathogens.
README: Nutribloods: Novel synthetic lepidopteran haemolymphs for understanding insect-microbe nutritional interactions in vitro
https://doi.org/10.5061/dryad.wm37pvmz0
Description of the data and file structure
These data were generated from experiments designed to establish how bacterial growth (Xenorhabdus nematophila) varies in 20 synthetic haemolymphs that differ in their nutritional properties
Files and variables
File: dat2_archive.xlsx
Description: Bacterial growth in 20 synthetic haemolymphs varying in their nutritional properties.
Variables
- data are described in a 'Key' sheet in the Excel file
File: dat10_archive.xlsx
Description: Bacterial growth in synthetic haemolymphs in which all nutrients are constant across all haemolymphs except for one nutrient, carbohydrate, protein or amino acids.
Variables
- data are described in a 'Key' sheet in the Excel file
Code/software
All data are in Excel files
Access information
Other publicly accessible locations of the data:
- n/a
Data was derived from the following sources:
- n/a
Methods
All data were collected in a laboratory setting