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Predictors of genomic differentiation within a hybrid taxon

Citation

Cuevas, Angelica et al. (2022), Predictors of genomic differentiation within a hybrid taxon, Dryad, Dataset, https://doi.org/10.5061/dryad.wpzgmsbns

Abstract

Hybridization is increasingly recognized as an important evolutionary force. Novel genetic methods now enable us to address how the genomes of parental species are combined in hybrid lineages. However, we still do not know the relative importance of admixed proportions, genome architecture and local selection in shaping hybrid genomes. Here, we take advantage of the genetically divergent island populations of Italian sparrow on Crete, Corsica and Sicily to investigate the predictors of genomic variation within a hybrid taxon. We test if differentiation is affected by recombination rate, selection, or variation in ancestry proportions. We find that the relationship between recombination rate and differentiation is less pronounced within hybrid lineages than between the parent species, as expected if purging of minor parent ancestry in low recombination regions reduces the variation available for differentiation. In addition, we find that differentiation between islands is correlated with differences in signatures of selection in two out of three comparisons. Signatures of selection within islands are correlated across all islands, suggesting that shared selection may mould genomic differentiation. The best predictor of strong differentiation within islands is the degree of differentiation from house sparrow, and hence loci with Spanish sparrow ancestry may vary more freely. Jointly, this suggests that constraints and selection interact in shaping the genomic landscape of differentiation in this hybrid species.

Methods

Genomic DNA was purified from blood samples. Double digestion of the genomic DNA for ddRAD using EcoR I and MseI restriction enzymes. Library pools were size selected for fragments between 500-600bp.  Illumina Nextseq500 machine and the 1x75bp sequencing format. Vcftools, plink, bcftools, GATK, among others programes were use for filtering and generation of a final master VCF file.

Here we provide scripts for processing raw data and for the statistical analysis in the study, VCF files and intermediate files used by the R script needed for the statistical analysis are also provided. 

Funding

Norwegian Research Council, Award: 240557

The Swedish Research Council and the European Union Marie Sklodowska Curie Action, Award: 2011- 302504

Wenner-Gren fellowship