Data from: Integrating variation in bacterial-fungal co-occurrence network with soil carbon dynamics
Data files
Oct 11, 2023 version files 35.14 KB
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Microbial_biomass.xlsx
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README.md
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SOC_Carbonmineralization.xlsx
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Soil_enzymatic_activity.xlsx
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soil_properties.csv
Abstract
Bacteria and fungi are core microorganisms in diverse ecosystems, and their cross-kingdom interactions are considered key determinants of microbiome structure and ecosystem functioning. However, how bacterial-fungal interactions mediate soil organic carbon (SOC) dynamics remains largely unexplored in the context of artificial forest ecosystems. Here, we characterized soil bacterial and fungal communities in four successive planting of Eucalyptus and compared them to a neighboring evergreen broadleaf forest. Carbon (C) mineralization combined with five C-degrading enzymatic activities was investigated to determine the effects of successive planting of Eucalyptus on SOC dynamics. Our results indicated that successive planting of Eucalyptus significantly altered the diversity and structure of soil bacterial and fungal communities and increased the negative bacterial-fungal associations. The bacterial diversity significantly decreased in all Eucalyptus plantations compared to the evergreen forest, while the fungal diversity showed the opposite trend. The ratio of negative bacterial-fungal associations increased with successive planting of Eucalyptus due to the decrease in SOC, ammonia nitrogen (NH4+−N), nitrate nitrogen (NO3−−N), and available phosphorus (AP). Structural equation modeling indicated that the potential cross-kingdom competition, based on the ratio of negative bacterial-fungal correlations, was significantly negatively associated with the diversity of total bacteria and keystone bacteria, thereby increasing C-degrading enzymatic activities and C mineralization.
Synthesis and applications: Our results highlight the regulatory role of the negative bacterial-fungal association in enhancing the correlation between bacterial diversity and C mineralization. This suggests that promoting short-term successive planting in the management of Eucalyptus plantations can mitigate the impact of this association on SOC decomposition. Taken together, our study advances the understanding of bacterial-fungal negative associations to mediate carbon mineralization in Eucalyptus plantations, giving us a new insight into SOC cycling dynamics in artificial forests.
README
The data is for the manuscript "Integrating variation in bacterial-fungal co-occurrence network with soil carbon dynamics".
Author: Lijun Chen, Francisco Dini-Andreote, Hongqiang Liu, Huaxiang Wang, Alex Dumbrell, Zhengye Wang, Xingyu Chen, Fangfang Chen, Xiaolong Chen,Lichao Wu, Yuji Jiang*
*Email of corresponding author: yjjiang@issas.ac.cn
In the "soil_properties"data, the meaning of each cloumn is below:
"pH" is the soil pH determined with a soil:water ratio of 1:2.5.
"NH4+−N" is the soil ammonium nitrogen content. The unit is milligram per kilogram.
"NO3−−N" is the soil nitrate nitrogen content. The unit is milligram per kilogram.
"TN" is the soil total nitrogen content. The unit is gram per kilogram.
"TP" is the soil total phosphorus content. The unit is gram per kilogram.
"TK" is the soil total potassium content. The unit is gram per kilogram.
"AP" is the soil available phosphorus content. The unit is milligram per kilogram.
"AK" is the soil available potassium content. The unit is milligram per kilogram.
"CEC" is the soil cation exchange capacity. The unit is centimole per kilogram.
"BD" is the soil bulk density. The unit is gram per cubic centimetre.
"Pt" is the soil total porosity. The unit is %.
"Pa" is the soil aeration porosity. The unit is %.
In the "SOC_Carbonmineralization" data, the meaning of each cloumn is below:
"SOC" is the soil organic carbon content. The unit is gram per kilogram.
"Carbon mineralization" is the soil carbon mineralization rate. The unit is milligram carbon per kilogram soil per hour.
In the "Mocrobial_biomass" data, the meaning of each cloumn is below:
"MBC" is the soil microbial biomass carbon. The unit is milligram per kilogram.
"MBN" is the soil microbial biomass nitrogen. The unit is milligram per kilogram.
"MBP" is the soil microbial biomass phosphorus. The unit is milligram per kilogram.
In the "Soil_enzymatic_activity" data, the meaning of each cloumn is below:
"Units" is the unit for each enzyme.
"β-glucosidase" represent the activity of soil β-1,4-glucosidase (EC 3.2.1.21).
"β-xylosidase" represent the activity of soil β-xylosidase (EC 3.2.1.37).
"Acid phosphatase" represent the activity of soil acid phosphatase (EC 3.1.3.2).
"N-acetyl glucosaminidase" represent the activity of soil β-N-acetylglucosaminidase (EC 3.1.6.1).
"Sucrase" represent the activity of soil sucrase (EC 3.2.1.26).
"Total" is the arithmetic sum of the above five ezymatic activity.
"CK1", "CK2" and "CK3" are replicaties of evergreen broadleaf forest control (CK) in the field experiment.
"PrG1", "PrG2" and "PrG3" are replicaties of the first generation (PrG) of Eucalyptus reforestation in the field experiment.
"SeG1", "SeG2" and "SeG3" are replicaties of the second generation (SeG) of Eucalyptus reforestation in the field experiment.
"ThG1", "ThG2" and "ThG3" are replicaties of the third generation (ThG) of Eucalyptus reforestation in the field experiment.
"FoG1", "FoG2" and "FoG3" are replicaties of the forth generation (FoG) of Eucalyptus reforestation in the field experiment.
Methods
The experimental site is located in the state-owned Daguishan Forest Farm in Hezhou City, Guangxi Zhuang Autonomous Region, China (111°20’5’’E, 23°58’33’’N). The mean annual temperature in this area is 19.3℃, with mean annual precipitation and evaporation of 2,056 mm and 1,200 mm, respectively. The soil type is classified as red soil (i.e., ferralsols). A total of 12 plots (20 m wide × 30 m long) were established to collect soil samples in triplicate representing four generations of Eucalyptus plantation. In each treatment, the Eucalyptus trees were at the same stage of development (i.e., 4 years after planting). The treatments included the first generation (PrG) of Eucalyptus reforestation, the second generation (SeG) regenerating after the PrG was cut, the third generation (ThG) regenerating after the SeG, and the fourth generation (FoG) regenerating after the ThG. An evergreen broadleaf forest with three adjacent plots was selected as the control (CK), which was a precursor to the Eucalyptus plantation. All the plots were located within a 5 km2 area. The Eucalyptus species planted in these plots was a hybrid of Eucalyptus urophylla S.T. Blake × Eucalyptus grandis Hill ex Maiden (Eucalyptus urograndis).