Targeting gravid females through chemical lures is a promising strategy in vector control; however, it requires the understanding of the factors susceptible to alter female oviposition behaviour. Here, we evaluated the effect of infection with chikungunya virus (CHIKV) and the number of gonotrophic cycles (GCs) on oviposition activity in Ae. aegypti. Dual choice oviposition assays were performed, where dodecanoic acid, pentadecanoic acid, n-heneicosane and a Sargasssum fluitans (Børgesen) Børgesen extract were tested in uninfected females and females infected with CHIKV, at the 1^st and 2^nd GC. Infected females displayed a lower percentage of oviposition and a higher number of eggs laid at the 1^st GC. Then, combined effects of GC and CHIKV induced the preference towards the tested candidates to either increase or decrease with a chemical-dependent effect. These results allow a deeper understanding of the mechanisms involved in oviposition site selection and highlight the need for taking into account physiological stage changes to increase the control programs efficacy.