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Mass spectrometry data of Chalmydomonas reinhardtii central pair mutants

Citation

Bui, Khanh Huy et al. (2020), Mass spectrometry data of Chalmydomonas reinhardtii central pair mutants, Dryad, Dataset, https://doi.org/10.5061/dryad.x0k6djhg6

Abstract

The following data includes the mass spectrometry results from 5 different strains of Chlamydomonas reinhardtii. The first strain is CC-124 or more commonly referred to as the wild type strain. The second strain is a mutant that lacks the central pair pf15. Other central pair mutants are pf16, pf6 and cpc1.

All strains were cultured under identical conditions and the doublet microtubules were purified and treated twice with NaCl before mass spectrometry. Biological triplicates were performed on each strain for mass spectrometry.

Methods

Cilia were purified from Chlamydomonas, salt-treated to remove outside proteins. The samples were subjected to tandem mass spectrometry according to the protocol described in Dai et al. 2019, bioRxiv. (https://doi.org/10.1101/739383).

Usage Notes

Mass Spectrometry Core Data File (.sf3 file) - can be opened using the Proteome Software Scaffold free viewer (http://www.proteomesoftware.com/products/scaffold/download/).

Funding

Natural Sciences and Engineering Research Council of Canada, Award: RGPIN-2016-04954

Canadian Institutes of Health Research, Award: PJT-156354