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Functional interactions among neurons within single columns of macaque V1

Citation

Trepka, Ethan et al. (2022), Functional interactions among neurons within single columns of macaque V1, Dryad, Dataset, https://doi.org/10.5061/dryad.x3ffbg7p2

Abstract

Recent developments in high-density neurophysiological tools now make it possible to record from hundreds of single neurons within local, highly interconnected neural networks. Among the many advantages of such recordings is that they dramatically increase the quantity of identifiable, functional interactions between neurons thereby providing an unprecedented view of local circuits. Using high-density, Neuropixels recordings from single neocortical columns of primary visual cortex in nonhuman primates, we identified 1000s of functionally interacting neuronal pairs using established crosscorrelation approaches. Our results reveal clear and systematic variations in the synchrony and strength of functional interactions within single cortical columns. Despite neurons residing within the same column, both measures of interactions depended heavily on the vertical distance separating neuronal pairs, as well as on the similarity of stimulus tuning. In addition, we leveraged the statistical power afforded by the large numbers of functionally interacting pairs to categorize interactions between neurons based on their crosscorrelation functions. These analyses identified distinct, putative classes of functional interactions within the full population. These classes of functional interactions were corroborated by their unique distributions across defined laminar compartments and were consistent with known properties of V1 cortical circuitry, such as the lead-lag relationship between simple and complex cells. Our results provide a clear proof-of-principle for the use of high-density neurophysiological recordings to assess circuit-level interactions within local neuronal networks.

Methods

The activity of V1 neurons was recorded in two anesthetized macaque monkeys (M1, M2) using high-density, multi-contact Neuropixel probes (version 3A; IMEC Inc, Belgium). Probes were inserted into the lateral operculum of V1 with the aid of a surgical microscope at angles nearly perpendicular to the cortical surface. In each of the 5 experimental sessions (3 in M1, 2 in M2), we measured the visual responses of 115–221 simultaneously recorded neurons to drifting gratings presented at varying orientations (total = 802 neurons). To estimate the functional interactions between pairs of neurons recorded simultaneously within columns of V1, we computed cross-correlograms (CCGs) using the 802 visually responsive neurons recorded across sessions. CCGs were computed from the spike trains of 68,579 pairs of simultaneously recorded neurons (6,555–24,310 pairs/session).

Usage Notes

Software of MATLAB (The MathWorks Inc.) is required to open the data files. Users may register and download the software at https://www.mathworks.com

Funding

National Eye Institute, Award: EY014924

National Institute of Neurological Disorders and Stroke, Award: NS116623

National Eye Institute, Award: EY029759