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Dryad

Nematode community dynamics in Johnson Su compost

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Jan 20, 2025 version files 12.05 KB

Abstract

Characterising microscopic communities is important for both ecological research and diagnostic applications. In these areas, light microscopy is still a fundamental tool due to its accessibility and real-time imaging capabilities. However, the higher magnifications required for accurate identifications typically necessitates extensive sample preparation. Therefore, this study aimed to design and validate a bright field light microscopy-based platform with magnification up to 1000x without the need for extensive sample preparation. An adapted version of the Peter’s slide was developed, which allows the use of specialised long-working distance objectives without the need for oil immersion. Image quality at higher magnifications were evaluated using nematodes as test organisms. Additionally, the use of the adapted Peter’s slide in characterising microscopic communities was evaluated by studying the temporal dynamics in nematode functional diversity from Johnson Su compost. The adapted Peter’s slide facilitated the identification and counting of nematodes to genus level, demonstrating sufficient image clarity even at 1000x magnification. My results confirmed that this platform overcomes the limitations of standard approaches by eliminating the need for extensive sample preparation and mounting on microscope slides, which are typically required for high-magnification observations. This platform reduces sample processing time, while also facilitating live viewing under high magnification. These features also make it a valuable educational tool in classroom settings. Future enhancements could include the integration of AI-based imaging software to automate and refine identification and counting processes. However, a current limitation is the cost of the long-working objectives, which may limit widespread adoption.