Estuarine fishes associated with intertidal oyster reefs characterised using environmental DNA and baited remote underwater video
Data files
Feb 10, 2021 version files 3.28 GB
-
Extraction_16SFish_Mar19_120.fastq
5.95 KB
-
Extraction_16SNest_Aug19_151.fastq
522.90 KB
-
Extraction_16SNest_Mar19_120.fastq
1.26 MB
-
Extraction_Fish16S_Aug19_151.fastq
859 B
-
Extraction_MiFishUAug19_151.fastq
1.08 KB
-
Filtration_16SFish_Mar19_119.fastq
845 B
-
Filtration_16SNest_Mar19_121.fastq
722 B
-
Neg1_16SNest_Mar19.fastq
901 B
-
Neg1_MiFishU_Mar19.fastq
55.20 KB
-
Neg1.fastq
1.04 KB
-
Neg2_16SNest_Mar19.fastq
21.46 KB
-
Oyster1_16SFish_Mar19_89.fastq
31.58 MB
-
Oyster1_16SFish_Mar19_90.fastq
25.43 MB
-
Oyster1_16SFish_Mar19_91.fastq
22.50 MB
-
Oyster1_16SFish_Mar19_92.fastq
26.29 MB
-
Oyster1_16SFish_Mar19_93.fastq
19.07 MB
-
Oyster1_16SFish_Mar19_94.fastq
15.34 MB
-
Oyster1_16SNest_Aug19_121.fastq
9.20 MB
-
Oyster1_16SNest_Aug19_122.fastq
17.60 MB
-
Oyster1_16SNest_Aug19_123.fastq
10.43 MB
-
Oyster1_16SNest_Aug19_124.fastq
11.09 MB
-
Oyster1_16SNest_Aug19_125.fastq
9.98 MB
-
Oyster1_16SNest_Aug19_126.fastq
12.84 MB
-
Oyster1_16SNest_Mar19_89.fastq
28.43 MB
-
Oyster1_16SNest_Mar19_90.fastq
27.42 MB
-
Oyster1_16SNest_Mar19_91.fastq
22.81 MB
-
Oyster1_16SNest_Mar19_92.fastq
21.82 MB
-
Oyster1_16SNest_Mar19_93.fastq
14.49 MB
-
Oyster1_16SNest_Mar19_94.fastq
16.04 MB
-
Oyster1_Fish16S_Aug19_121.fastq
10.74 MB
-
Oyster1_Fish16S_Aug19_122.fastq
15.58 MB
-
Oyster1_Fish16S_Aug19_123.fastq
16.75 MB
-
Oyster1_Fish16S_Aug19_124.fastq
12.28 MB
-
Oyster1_Fish16S_Aug19_125.fastq
16.93 MB
-
Oyster1_Fish16S_Aug19_126.fastq
9.95 MB
-
Oyster1_MiFishU_Aug19_121.fastq
26.42 MB
-
Oyster1_MiFishU_Aug19_122.fastq
24.90 MB
-
Oyster1_MiFishU_Aug19_123.fastq
22.36 MB
-
Oyster1_MiFishU_Aug19_124.fastq
15.60 MB
-
Oyster1_MiFishU_Aug19_125.fastq
15.97 MB
-
Oyster1_MiFishU_Aug19_126.fastq
12.48 MB
-
Oyster1_MiFishU_Mar19_89.fastq
33.19 MB
-
Oyster1_MiFishU_Mar19_90.fastq
38.75 MB
-
Oyster1_MiFishU_Mar19_91.fastq
29.79 MB
-
Oyster1_MiFishU_Mar19_92.fastq
29.35 MB
-
Oyster1_MiFishU_Mar19_93.fastq
19.57 MB
-
Oyster1_MiFishU_Mar19_94.fastq
19.72 MB
-
Oyster2_16SFish_Mar19_113.fastq
32.19 MB
-
Oyster2_16SFish_Mar19_114.fastq
43.66 MB
-
Oyster2_16SFish_Mar19_115.fastq
41.93 MB
-
Oyster2_16SFish_Mar19_116.fastq
41.70 MB
-
Oyster2_16SFish_Mar19_117.fastq
33.38 MB
-
Oyster2_16SFish_Mar19_118.fastq
46.99 MB
-
Oyster2_16SNest_Aug19_145.fastq
9.44 MB
-
Oyster2_16SNest_Aug19_146.fastq
9.69 MB
-
Oyster2_16SNest_Aug19_147.fastq
22.44 MB
-
Oyster2_16SNest_Aug19_148.fastq
29.01 MB
-
Oyster2_16SNest_Aug19_149.fastq
7.35 MB
-
Oyster2_16SNest_Aug19_150.fastq
5.80 MB
-
Oyster2_16SNest_Mar19_113.fastq
20.41 MB
-
Oyster2_16SNest_Mar19_114.fastq
25.46 MB
-
Oyster2_16SNest_Mar19_115.fastq
23.46 MB
-
Oyster2_16SNest_Mar19_116.fastq
27.50 MB
-
Oyster2_16SNest_Mar19_117.fastq
17.46 MB
-
Oyster2_16SNest_Mar19_118.fastq
15.95 MB
-
Oyster2_Fish16S_Aug19_145.fastq
21 MB
-
Oyster2_Fish16S_Aug19_146.fastq
16.05 MB
-
Oyster2_Fish16S_Aug19_147.fastq
5.79 MB
-
Oyster2_Fish16S_Aug19_148.fastq
17.35 MB
-
Oyster2_Fish16S_Aug19_149.fastq
14.04 MB
-
Oyster2_Fish16S_Aug19_150.fastq
20.76 MB
-
Oyster2_MiFishU_Aug19_145.fastq
15.33 MB
-
Oyster2_MiFishU_Aug19_146.fastq
18.94 MB
-
Oyster2_MiFishU_Aug19_147.fastq
18.72 MB
-
Oyster2_MiFishU_Aug19_148.fastq
21.35 MB
-
Oyster2_MiFishU_Aug19_149.fastq
25.50 MB
-
Oyster2_MiFishU_Aug19_150.fastq
15.95 MB
-
Oyster2_MiFishU_Mar19_113.fastq
27.55 MB
-
Oyster2_MiFishU_Mar19_114.fastq
33.75 MB
-
Oyster2_MiFishU_Mar19_115.fastq
30.40 MB
-
Oyster2_MiFishU_Mar19_116.fastq
33.83 MB
-
Oyster2_MiFishU_Mar19_117.fastq
25.76 MB
-
Oyster2_MiFishU_Mar19_118.fastq
37.37 MB
-
Pos_16SNest_Aug19.fastq
8.91 MB
-
Pos_MiFishU_Aug19.fastq
43.69 MB
-
Sand1_16SFish_Mar19_107.fastq
40.95 MB
-
Sand1_16SFish_Mar19_108.fastq
54.54 MB
-
Sand1_16SFish_Mar19_109.fastq
33.67 MB
-
Sand1_16SFish_Mar19_110.fastq
27.67 MB
-
Sand1_16SFish_Mar19_111.fastq
22.56 MB
-
Sand1_16SFish_Mar19_112.fastq
26.44 MB
-
Sand1_16SNest_Aug19_139.fastq
13.24 MB
-
Sand1_16SNest_Aug19_140.fastq
7.85 MB
-
Sand1_16SNest_Aug19_141.fastq
19.95 MB
-
Sand1_16SNest_Aug19_142.fastq
17.13 MB
-
Sand1_16SNest_Aug19_143.fastq
11 MB
-
Sand1_16SNest_Aug19_144.fastq
9.42 MB
-
Sand1_16SNest_Mar19_107.fastq
18.74 MB
-
Sand1_16SNest_Mar19_108.fastq
37.62 MB
-
Sand1_16SNest_Mar19_109.fastq
18.77 MB
-
Sand1_16SNest_Mar19_110.fastq
21.70 MB
-
Sand1_16SNest_Mar19_111.fastq
21.23 MB
-
Sand1_16SNest_Mar19_112.fastq
19.32 MB
-
Sand1_Fish16S_Aug19_139.fastq
11.54 MB
-
Sand1_Fish16S_Aug19_140.fastq
15.26 MB
-
Sand1_Fish16S_Aug19_141.fastq
16.36 MB
-
Sand1_Fish16S_Aug19_142.fastq
8.15 MB
-
Sand1_Fish16S_Aug19_143.fastq
22.01 MB
-
Sand1_Fish16S_Aug19_144.fastq
21.57 MB
-
Sand1_MiFishU_Aug19_139.fastq
15.98 MB
-
Sand1_MiFishU_Aug19_140.fastq
18.43 MB
-
Sand1_MiFishU_Aug19_141.fastq
18.51 MB
-
Sand1_MiFishU_Aug19_142.fastq
20.50 MB
-
Sand1_MiFishU_Aug19_143.fastq
24.41 MB
-
Sand1_MiFishU_Aug19_144.fastq
19.76 MB
-
Sand1_MiFishU_Mar19_107.fastq
35.48 MB
-
Sand1_MiFishU_Mar19_108.fastq
40.61 MB
-
Sand1_MiFishU_Mar19_109.fastq
31.32 MB
-
Sand1_MiFishU_Mar19_110.fastq
36.57 MB
-
Sand1_MiFishU_Mar19_111.fastq
37.78 MB
-
Sand1_MiFishU_Mar19_112.fastq
27.95 MB
-
Sand2_16SFish_Mar19_101.fastq
23.63 MB
-
Sand2_16SFish_Mar19_102.fastq
27.16 MB
-
Sand2_16SFish_Mar19_103.fastq
44.06 MB
-
Sand2_16SFish_Mar19_104.fastq
50.39 MB
-
Sand2_16SFish_Mar19_105.fastq
39.82 MB
-
Sand2_16SFish_Mar19_106.fastq
42.36 MB
-
Sand2_16SNest_Aug19_133.fastq
15.95 MB
-
Sand2_16SNest_Aug19_134.fastq
11.23 MB
-
Sand2_16SNest_Aug19_135.fastq
20.96 MB
-
Sand2_16SNest_Aug19_136.fastq
16.12 MB
-
Sand2_16SNest_Aug19_137.fastq
15.36 MB
-
Sand2_16SNest_Aug19_138.fastq
24.87 MB
-
Sand2_16SNest_Mar19_101.fastq
23.74 MB
-
Sand2_16SNest_Mar19_102.fastq
19.67 MB
-
Sand2_16SNest_Mar19_103.fastq
18.92 MB
-
Sand2_16SNest_Mar19_104.fastq
19.85 MB
-
Sand2_16SNest_Mar19_105.fastq
12.45 MB
-
Sand2_16SNest_Mar19_106.fastq
8.65 MB
-
Sand2_Fish16S_Aug19_133.fastq
7.49 MB
-
Sand2_Fish16S_Aug19_134.fastq
9.92 MB
-
Sand2_Fish16S_Aug19_135.fastq
14.27 MB
-
Sand2_Fish16S_Aug19_136.fastq
10.13 MB
-
Sand2_Fish16S_Aug19_137.fastq
19.36 MB
-
Sand2_Fish16S_Aug19_138.fastq
13.04 MB
-
Sand2_MiFishU_Aug19_133.fastq
17.24 MB
-
Sand2_MiFishU_Aug19_134.fastq
21.21 MB
-
Sand2_MiFishU_Aug19_135.fastq
26.02 MB
-
Sand2_MiFishU_Aug19_136.fastq
21.50 MB
-
Sand2_MiFishU_Aug19_137.fastq
20.40 MB
-
Sand2_MiFishU_Aug19_138.fastq
18.66 MB
-
Sand2_MiFishU_Mar19_101.fastq
34.67 MB
-
Sand2_MiFishU_Mar19_102.fastq
28.48 MB
-
Sand2_MiFishU_Mar19_103.fastq
31.30 MB
-
Sand2_MiFishU_Mar19_104.fastq
31.21 MB
-
Sand2_MiFishU_Mar19_105.fastq
26.50 MB
-
Sand2_MiFishU_Mar19_106.fastq
30.71 MB
Feb 12, 2021 version files 3.28 GB
-
BRUV_data.xlsx
15.49 KB
-
Extraction_16SFish_Mar19_120.fastq
5.95 KB
-
Extraction_16SNest_Aug19_151.fastq
522.90 KB
-
Extraction_16SNest_Mar19_120.fastq
1.26 MB
-
Extraction_Fish16S_Aug19_151.fastq
859 B
-
Extraction_MiFishUAug19_151.fastq
1.08 KB
-
Filtration_16SFish_Mar19_119.fastq
845 B
-
Filtration_16SNest_Mar19_121.fastq
722 B
-
Neg1_16SNest_Mar19.fastq
901 B
-
Neg1_MiFishU_Mar19.fastq
55.20 KB
-
Neg1.fastq
1.04 KB
-
Neg2_16SNest_Mar19.fastq
21.46 KB
-
Oyster1_16SFish_Mar19_89.fastq
31.58 MB
-
Oyster1_16SFish_Mar19_90.fastq
25.43 MB
-
Oyster1_16SFish_Mar19_91.fastq
22.50 MB
-
Oyster1_16SFish_Mar19_92.fastq
26.29 MB
-
Oyster1_16SFish_Mar19_93.fastq
19.07 MB
-
Oyster1_16SFish_Mar19_94.fastq
15.34 MB
-
Oyster1_16SNest_Aug19_121.fastq
9.20 MB
-
Oyster1_16SNest_Aug19_122.fastq
17.60 MB
-
Oyster1_16SNest_Aug19_123.fastq
10.43 MB
-
Oyster1_16SNest_Aug19_124.fastq
11.09 MB
-
Oyster1_16SNest_Aug19_125.fastq
9.98 MB
-
Oyster1_16SNest_Aug19_126.fastq
12.84 MB
-
Oyster1_16SNest_Mar19_89.fastq
28.43 MB
-
Oyster1_16SNest_Mar19_90.fastq
27.42 MB
-
Oyster1_16SNest_Mar19_91.fastq
22.81 MB
-
Oyster1_16SNest_Mar19_92.fastq
21.82 MB
-
Oyster1_16SNest_Mar19_93.fastq
14.49 MB
-
Oyster1_16SNest_Mar19_94.fastq
16.04 MB
-
Oyster1_Fish16S_Aug19_121.fastq
10.74 MB
-
Oyster1_Fish16S_Aug19_122.fastq
15.58 MB
-
Oyster1_Fish16S_Aug19_123.fastq
16.75 MB
-
Oyster1_Fish16S_Aug19_124.fastq
12.28 MB
-
Oyster1_Fish16S_Aug19_125.fastq
16.93 MB
-
Oyster1_Fish16S_Aug19_126.fastq
9.95 MB
-
Oyster1_MiFishU_Aug19_121.fastq
26.42 MB
-
Oyster1_MiFishU_Aug19_122.fastq
24.90 MB
-
Oyster1_MiFishU_Aug19_123.fastq
22.36 MB
-
Oyster1_MiFishU_Aug19_124.fastq
15.60 MB
-
Oyster1_MiFishU_Aug19_125.fastq
15.97 MB
-
Oyster1_MiFishU_Aug19_126.fastq
12.48 MB
-
Oyster1_MiFishU_Mar19_89.fastq
33.19 MB
-
Oyster1_MiFishU_Mar19_90.fastq
38.75 MB
-
Oyster1_MiFishU_Mar19_91.fastq
29.79 MB
-
Oyster1_MiFishU_Mar19_92.fastq
29.35 MB
-
Oyster1_MiFishU_Mar19_93.fastq
19.57 MB
-
Oyster1_MiFishU_Mar19_94.fastq
19.72 MB
-
Oyster2_16SFish_Mar19_113.fastq
32.19 MB
-
Oyster2_16SFish_Mar19_114.fastq
43.66 MB
-
Oyster2_16SFish_Mar19_115.fastq
41.93 MB
-
Oyster2_16SFish_Mar19_116.fastq
41.70 MB
-
Oyster2_16SFish_Mar19_117.fastq
33.38 MB
-
Oyster2_16SFish_Mar19_118.fastq
46.99 MB
-
Oyster2_16SNest_Aug19_145.fastq
9.44 MB
-
Oyster2_16SNest_Aug19_146.fastq
9.69 MB
-
Oyster2_16SNest_Aug19_147.fastq
22.44 MB
-
Oyster2_16SNest_Aug19_148.fastq
29.01 MB
-
Oyster2_16SNest_Aug19_149.fastq
7.35 MB
-
Oyster2_16SNest_Aug19_150.fastq
5.80 MB
-
Oyster2_16SNest_Mar19_113.fastq
20.41 MB
-
Oyster2_16SNest_Mar19_114.fastq
25.46 MB
-
Oyster2_16SNest_Mar19_115.fastq
23.46 MB
-
Oyster2_16SNest_Mar19_116.fastq
27.50 MB
-
Oyster2_16SNest_Mar19_117.fastq
17.46 MB
-
Oyster2_16SNest_Mar19_118.fastq
15.95 MB
-
Oyster2_Fish16S_Aug19_145.fastq
21 MB
-
Oyster2_Fish16S_Aug19_146.fastq
16.05 MB
-
Oyster2_Fish16S_Aug19_147.fastq
5.79 MB
-
Oyster2_Fish16S_Aug19_148.fastq
17.35 MB
-
Oyster2_Fish16S_Aug19_149.fastq
14.04 MB
-
Oyster2_Fish16S_Aug19_150.fastq
20.76 MB
-
Oyster2_MiFishU_Aug19_145.fastq
15.33 MB
-
Oyster2_MiFishU_Aug19_146.fastq
18.94 MB
-
Oyster2_MiFishU_Aug19_147.fastq
18.72 MB
-
Oyster2_MiFishU_Aug19_148.fastq
21.35 MB
-
Oyster2_MiFishU_Aug19_149.fastq
25.50 MB
-
Oyster2_MiFishU_Aug19_150.fastq
15.95 MB
-
Oyster2_MiFishU_Mar19_113.fastq
27.55 MB
-
Oyster2_MiFishU_Mar19_114.fastq
33.75 MB
-
Oyster2_MiFishU_Mar19_115.fastq
30.40 MB
-
Oyster2_MiFishU_Mar19_116.fastq
33.83 MB
-
Oyster2_MiFishU_Mar19_117.fastq
25.76 MB
-
Oyster2_MiFishU_Mar19_118.fastq
37.37 MB
-
Pos_16SNest_Aug19.fastq
8.91 MB
-
Pos_MiFishU_Aug19.fastq
43.69 MB
-
Sand1_16SFish_Mar19_107.fastq
40.95 MB
-
Sand1_16SFish_Mar19_108.fastq
54.54 MB
-
Sand1_16SFish_Mar19_109.fastq
33.67 MB
-
Sand1_16SFish_Mar19_110.fastq
27.67 MB
-
Sand1_16SFish_Mar19_111.fastq
22.56 MB
-
Sand1_16SFish_Mar19_112.fastq
26.44 MB
-
Sand1_16SNest_Aug19_139.fastq
13.24 MB
-
Sand1_16SNest_Aug19_140.fastq
7.85 MB
-
Sand1_16SNest_Aug19_141.fastq
19.95 MB
-
Sand1_16SNest_Aug19_142.fastq
17.13 MB
-
Sand1_16SNest_Aug19_143.fastq
11 MB
-
Sand1_16SNest_Aug19_144.fastq
9.42 MB
-
Sand1_16SNest_Mar19_107.fastq
18.74 MB
-
Sand1_16SNest_Mar19_108.fastq
37.62 MB
-
Sand1_16SNest_Mar19_109.fastq
18.77 MB
-
Sand1_16SNest_Mar19_110.fastq
21.70 MB
-
Sand1_16SNest_Mar19_111.fastq
21.23 MB
-
Sand1_16SNest_Mar19_112.fastq
19.32 MB
-
Sand1_Fish16S_Aug19_139.fastq
11.54 MB
-
Sand1_Fish16S_Aug19_140.fastq
15.26 MB
-
Sand1_Fish16S_Aug19_141.fastq
16.36 MB
-
Sand1_Fish16S_Aug19_142.fastq
8.15 MB
-
Sand1_Fish16S_Aug19_143.fastq
22.01 MB
-
Sand1_Fish16S_Aug19_144.fastq
21.57 MB
-
Sand1_MiFishU_Aug19_139.fastq
15.98 MB
-
Sand1_MiFishU_Aug19_140.fastq
18.43 MB
-
Sand1_MiFishU_Aug19_141.fastq
18.51 MB
-
Sand1_MiFishU_Aug19_142.fastq
20.50 MB
-
Sand1_MiFishU_Aug19_143.fastq
24.41 MB
-
Sand1_MiFishU_Aug19_144.fastq
19.76 MB
-
Sand1_MiFishU_Mar19_107.fastq
35.48 MB
-
Sand1_MiFishU_Mar19_108.fastq
40.61 MB
-
Sand1_MiFishU_Mar19_109.fastq
31.32 MB
-
Sand1_MiFishU_Mar19_110.fastq
36.57 MB
-
Sand1_MiFishU_Mar19_111.fastq
37.78 MB
-
Sand1_MiFishU_Mar19_112.fastq
27.95 MB
-
Sand2_16SFish_Mar19_101.fastq
23.63 MB
-
Sand2_16SFish_Mar19_102.fastq
27.16 MB
-
Sand2_16SFish_Mar19_103.fastq
44.06 MB
-
Sand2_16SFish_Mar19_104.fastq
50.39 MB
-
Sand2_16SFish_Mar19_105.fastq
39.82 MB
-
Sand2_16SFish_Mar19_106.fastq
42.36 MB
-
Sand2_16SNest_Aug19_133.fastq
15.95 MB
-
Sand2_16SNest_Aug19_134.fastq
11.23 MB
-
Sand2_16SNest_Aug19_135.fastq
20.96 MB
-
Sand2_16SNest_Aug19_136.fastq
16.12 MB
-
Sand2_16SNest_Aug19_137.fastq
15.36 MB
-
Sand2_16SNest_Aug19_138.fastq
24.87 MB
-
Sand2_16SNest_Mar19_101.fastq
23.74 MB
-
Sand2_16SNest_Mar19_102.fastq
19.67 MB
-
Sand2_16SNest_Mar19_103.fastq
18.92 MB
-
Sand2_16SNest_Mar19_104.fastq
19.85 MB
-
Sand2_16SNest_Mar19_105.fastq
12.45 MB
-
Sand2_16SNest_Mar19_106.fastq
8.65 MB
-
Sand2_Fish16S_Aug19_133.fastq
7.49 MB
-
Sand2_Fish16S_Aug19_134.fastq
9.92 MB
-
Sand2_Fish16S_Aug19_135.fastq
14.27 MB
-
Sand2_Fish16S_Aug19_136.fastq
10.13 MB
-
Sand2_Fish16S_Aug19_137.fastq
19.36 MB
-
Sand2_Fish16S_Aug19_138.fastq
13.04 MB
-
Sand2_MiFishU_Aug19_133.fastq
17.24 MB
-
Sand2_MiFishU_Aug19_134.fastq
21.21 MB
-
Sand2_MiFishU_Aug19_135.fastq
26.02 MB
-
Sand2_MiFishU_Aug19_136.fastq
21.50 MB
-
Sand2_MiFishU_Aug19_137.fastq
20.40 MB
-
Sand2_MiFishU_Aug19_138.fastq
18.66 MB
-
Sand2_MiFishU_Mar19_101.fastq
34.67 MB
-
Sand2_MiFishU_Mar19_102.fastq
28.48 MB
-
Sand2_MiFishU_Mar19_103.fastq
31.30 MB
-
Sand2_MiFishU_Mar19_104.fastq
31.21 MB
-
Sand2_MiFishU_Mar19_105.fastq
26.50 MB
-
Sand2_MiFishU_Mar19_106.fastq
30.71 MB
Abstract
It has been widely shown that oyster reefs enhance local biodiversity and fisheries production. Therefore, in this study fish assemblages from oyster reefs were compared to sandy habitats using environmental DNA (eDNA) metabarcoding and baited remote underwater video. Fish diversity from eDNA was characterised using three metabarcoding assays - two that target the mitochondrial 16S region and one that targets the 12S region. Fish assemblages differed with each assay, as well as to that detected by BRUVs. Overall, 112 fish genera were identified, with 78 more genera detected using eDNA metabarcoding than those observed with BRUVs. Both eDNA and BRUVs resolved a higher number of fish genera associated with oyster reefs than with sand sites, and a different fish composition between habitats was also resolved using each method. Furthermore, eDNA was shown to be useful towards characterising the gamma diversity of the estuary, due to the intertidal nature and hydrodynamics of the system, as well as the alpha diversity associated with oyster reefs and sand sites.
Methods
DNA was extracted from seawater samples collected from Port Stephens, NSW Australia.
The DNA was used in PCR using three independent metabarcoding assays (16SNest, Fish16S and MiFishU) to generate amplicon libraries.
Sequence data files were generated from the amplicon libraries on an Illumina MiSeq using a 300 cycle kit V2.
At the same sites as for eDNA, baited mini-BRUVs were deployed for 30 minutes.
Usage notes
Fastq files (i.e. list of DNA barcode sequences) for each sample and metabarcoding assay are provided.
Files names indicate the habitat (e.g. osyter) where the sample was collected from, followed by the site number (e.g. 1), metabarcoding assay (e.g. 16SFish), the date of collection (e.g. Mar19), and finally the unique sample identifier (e.g. 89).
For example: Oyster1_16SFish_Mar19_89.
Additional controls (negative, extraction, filtration) are also indicated in sample names.
In the excel datasheet, MaxN data of fish detections using BRUVs were generated with Event Measure. Habitat and site names for BRUV samples are consistent with the eDNA file names.