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Evolution of binding preferences among whole-genome duplicated transcription factors

Citation

Gera, Tamar; Jonas, Felix; More, Roye; Barkai, Naama (2022), Evolution of binding preferences among whole-genome duplicated transcription factors, Dryad, Dataset, https://doi.org/10.5061/dryad.xgxd254j6

Abstract

Throughout evolution, new transcription factors (TFs) emerge by gene duplication, promoting growth and rewiring of transcriptional networks. How TF duplicates diverge is known for only a few studied cases. To provide a genome-scale view, we considered the 35% of budding yeast TFs, classified as whole-genome duplication (WGD)-retained paralogs. Using high-resolution profiling, we find that ~60% of paralogs evolved differential binding preferences. We show that this divergence results primarily from variations outside the DNA binding domains (DBDs), while DBD preferences remain largely conserved. Analysis of non-WGD orthologs revealed that ancestral preferences are unevenly split between duplicates, while new targets are acquired preferentially by the least conserved paralog (biased sub/neo-functionalization). Dimer-forming paralogs evolved mostly one-sided dependency, while other paralogs interacted through low-magnitude DNA-binding competition that minimized paralog interference. We discuss the implications of our findings for the evolutionary design of transcriptional networks.

Methods

Next-generation Sequencing (NGS) data processed with MATLAB.

Usage Notes

This dataset is meant to be used with the scripts provided in the associated GitHub repo:
https://github.com/barkailab/Gera2021