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Data from: Oxidative stress in the retina and retinal pigment epithelium (RPE): role of aging, and DJ-1

Citation

Bonilha, Vera; Upadhya, Mala; Milliner, Caroline; Bell, Brent (2022), Data from: Oxidative stress in the retina and retinal pigment epithelium (RPE): role of aging, and DJ-1, Dryad, Dataset, https://doi.org/10.5061/dryad.xpnvx0kb6

Abstract

High levels of oxidative radicals generated by daily light exposure and high metabolic rate suggest that the antioxidant machinery of the retina and retinal pigment epithelium (RPE) is crucial for their survival. DJ-1 is a redox-sensitive protein that has been shown to have neuroprotective function in the brain in Parkinson’s disease and other neurodegenerative diseases. Here, we analyzed the role of DJ-1 in the retina during oxidative stress and aging. We induced low-level oxidative stress in young (3-month-old) and old (15-month-old) C57BL/6J (WT) and DJ-1 knockout (KO) mice and evaluated effects in the RPE and retina. Absence of DJ-1 resulted in increased retinal dysfunction in response to low levels of oxidative stress. Our findings suggest that loss of DJ-1 affects the RPE antioxidant machinery, rendering it unable to combat and neutralize low-level oxidative stress, irrespective of age. Moreover, they draw a parallel to the retinal degeneration observed in AMD, where the occurrence of genetic variants may leave the retina and RPE unable to fight sustained, low-levels of oxidative stress.

Funding

National Eye Institute, Award: National Institutes of Health R01EY027750

National Eye Institute, Award: P30EY025585

Research to Prevent Blindness, Award: Challenge Grant

Cleveland Clinic Foundation, Award: startup funds

National Eye Institute, Award: P30EY025585

National Institutes of Health, Award: 1S10RR031537-01

National Institutes of Health, Award: R01EY027750