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Dryad

Cinchona pubescens Transcriptome

O'Connor, Sarah1; Trenti, Francesco1; Grzech, Dagny1; Yamamoto, Kotaro1; Paetz, Christian1; Nakamura, Yoko1

Published Feb 23, 2021 on Dryad. https://doi.org/10.5061/dryad.z34tmpgcc

Data files

Feb 23, 2021 version files 271.63 MB

Abstract

The enzymatic basis for quinine 1 biosynthesis was investigated. Transcriptomic data from the producing plant Cinchona pubescens led to the discovery of three enzymes involved in early and late steps of the pathway. A medium-chain alcohol dehydrogenase (CpDCS) and an esterase (CpDCE) yielded the biosynthetic intermediate dihydrocorynantheal 2 from strictosidine aglycone 3. Additionally, the discovery of an O-methyltransferase specific for 6´-hydroxycinchoninone 4 suggested the final steps order to be cinchoninone 16/17 hydroxylation, methylation and keto-reduction.